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Journal article
Non-thermal dielectric barrier discharge plasma induces angiogenesis through reactive oxygen species
Journal of the Royal Society interface, v 9(66), pp 147-157
07 Jan 2012
PMID: 21653568
Abstract
Vascularization plays a key role in processes such as wound healing and tissue engineering. Non-thermal plasma, which primarily produces reactive oxygen species (ROS), has recently emerged as an efficient tool in medical applications including blood coagulation, sterilization and malignant cell apoptosis. Liquids and porcine aortic endothelial cells were treated with a non-thermal dielectric barrier discharge plasma in vitro. Plasma treatment of phosphate-buffered saline (PBS) and serum-free medium increased ROS concentration in a dose-dependent manner, with a higher concentration observed in serum-free medium compared with PBS. Species concentration inside cells peaked 1 h after treatment, followed by a decrease 3 h post treatment. Endothelial cells treated with a plasma dose of 4.2 J cm(-2) had 1.7 times more cells than untreated samples 5 days after plasma treatment. The 4.2 J cm(-2) plasma dose increased two-dimensional migration distance by 40 per cent compared with untreated control, while the number of cells that migrated through a three-dimensional collagen gel increased by 15 per cent. Tube formation was also enhanced by plasma treatment, with tube lengths in plasma-treated samples measuring 2.6 times longer than control samples. A fibroblast growth factor-2 (FGF-2) neutralizing antibody and ROS scavengers abrogated these angiogenic effects. These data indicate that plasma enhanced proliferation, migration and tube formation is due to FGF-2 release induced by plasma-produced ROS. Non-thermal plasma may be used as a potential tool for applying ROS in precise doses to enhance vascularization.
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Details
- Title
- Non-thermal dielectric barrier discharge plasma induces angiogenesis through reactive oxygen species
- Creators
- Krishna Priya Arjunan - Drexel UniversityGary Friedman - Drexel UniversityAlexander Fridman - Drexel UniversityAlisa Morss Clyne (Corresponding Author) - Drexel University
- Publication Details
- Journal of the Royal Society interface, v 9(66), pp 147-157
- Publisher
- The Royal Society
- Number of pages
- 11
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Electrical and Computer Engineering; C. and J. Nyheim Plasma Institute; Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000297322800014
- Scopus ID
- 2-s2.0-83455174205
- Other Identifier
- 991019169566104721
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- Web of Science research areas
- Biochemistry & Molecular Biology