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Journal article
Non-thermal plasma modulates cellular markers associated with immunogenicity in a model of latent HIV-1 infection
PloS one, v 16(3), pp e0247125-e0247125
01 Mar 2021
PMID: 33647028
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Effective control of infection by human immunodeficiency virus type 1 (HIV-1), the causative agent of the acquired immunodeficiency syndrome (AIDS), requires continuous and life-long use of anti-retroviral therapy (ART) by people living with HIV-1 (PLWH). In the absence of ART, HIV-1 reemergence from latently infected cells is ineffectively suppressed due to suboptimal innate and cytotoxic T lymphocyte responses. However, ART-free control of HIV-1 infection may be possible if the inherent immunological deficiencies can be reversed or restored. Herein we present a novel approach for modulating the immune response to HIV-1 that involves the use of non-thermal plasma (NTP), which is an ionized gas containing various reactive oxygen and nitrogen species (RONS). J-Lat cells were used as a model of latent HIV-1 infection to assess the effects of NTP application on viral latency and the expression of pro-phagocytic and pro-chemotactic damage-associated molecular patterns (DAMPs). Exposure of J-Lat cells to NTP resulted in stimulation of HIV-1 gene expression, indicating a role in latency reversal, a necessary first step in inducing adaptive immune responses to viral antigens. This was accompanied by the release of pro-inflammatory cytokines and chemokines including interleukin-1 beta (IL-1 beta) and interferon-gamma (IFN-gamma); the display of pro-phagocytic markers calreticulin (CRT), heat shock proteins (HSP) 70 and 90; and a correlated increase in macrophage phagocytosis of NTP-exposed J-Lat cells. In addition, modulation of surface molecules that promote or inhibit antigen presentation was also observed, along with an altered array of displayed peptides on MHC I, further suggesting methods by which NTP may modify recognition and targeting of cells in latent HIV-1 infection. These studies represent early progress toward an effective NTP-based ex vivo immunotherapy to resolve the dysfunctions of the immune system that enable HIV-1 persistence in PLWH.
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Details
- Title
- Non-thermal plasma modulates cellular markers associated with immunogenicity in a model of latent HIV-1 infection
- Creators
- Hager Mohamed - Drexel UniversityRamona Clemen - Leibniz Institute for Plasma Science and TechnologyEric Freund - Leibniz Institute for Plasma Science and TechnologyJan-Wilm Lackmann - University of CologneKristian Wende - Leibniz Institute for Plasma Science and TechnologyJennifer Connors - Drexel UniversityElias K. Haddad - Drexel UniversityWill Dampier - Drexel UniversityBrian Wigdahl - Drexel UniversityVandana Miller - Drexel UniversitySander Bekeschus - Leibniz Institute for Plasma Science and TechnologyFred C. Krebs - Drexel University
- Publication Details
- PloS one, v 16(3), pp e0247125-e0247125
- Publisher
- Public Library Science
- Number of pages
- 24
- Grant note
- Global Engagement Funding program of the Drexel University Office of Global Engagement and Education Abroad Department of Microbiology and Immunology Institute for Molecular Medicine and Infectious Disease at the Drexel University College of Medicine 03Z22DN11; 03Z22DN12; 03Z22Di1 / German Federal Ministry of Education and Research (BMBF); Federal Ministry of Education & Research (BMBF)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; Infectious Diseases (and HIV Medicine)
- Web of Science ID
- WOS:000625328100023
- Scopus ID
- 2-s2.0-85102332543
- Other Identifier
- 991019168852104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Immunology