Journal article
Nonlethal amphibian skin swabbing of cutaneous natural products for HPLC fingerprinting
Analytical methods, v 6(10), pp 3277-3284
01 Jan 2014
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Small organic molecules found on the skin of amphibians may help impart resistance to pathogens, such as the lethal fungus Batrachochytrium dendrobatidis. The study of these compounds has traditionally required euthanasia of the amphibian, followed by chemical extraction of excised skin. As an alternative method, we report the development and assessment of a non-lethal technique using foam-tipped swabs and HPLC analysis to directly isolate and characterize small molecules found on the skin of amphibians. This protocol was field-tested on Bioko Island, Equatorial Guinea with forty-seven frogs (representing 14 native species). Multiple species (particularly Afrixalus paradorsalis and Didynamipus sjostedti) carried sets of species-specific compounds (i.e., a chromatographic fingerprint). A principal coordinate analysis (PCO) of the commonly occurring compounds detected across all species revealed a significant relationship between chromatographic profile and species for all swab samples.
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Details
- Title
- Nonlethal amphibian skin swabbing of cutaneous natural products for HPLC fingerprinting
- Creators
- Thomas P. Umile - Villanova UniversityPatrick J. McLaughlin - Drexel UniversityKendall R. Johnson - Villanova UniversityShaya Honarvar - Drexel UniversityAlison L. Blackman - James Madison UniversityElizabeth A. Burzynski - Villanova UniversityRobert W. Davis - Villanova UniversityThais L. Teotonio - James Madison UniversityGail W. Hearn - Drexel UniversityChristine A. Hughey - James Madison UniversityAnthony F. Lagalante - Villanova UniversityKevin P. C. Minbiole - Villanova University
- Publication Details
- Analytical methods, v 6(10), pp 3277-3284
- Publisher
- Royal Soc Chemistry
- Number of pages
- 8
- Grant note
- Villanova University Mobil Equatorial Guinea Incorporated DEB-1136662 / NSF Dimensions of Biodiversity; National Science Foundation (NSF); NSF - Directorate for Biological Sciences (BIO) 1136662 / Division Of Environmental Biology; National Science Foundation (NSF); NSF - Directorate for Biological Sciences (BIO) la Universidad Nacional de Guinea Ecuatorial 96200-1-G279 / U.S. Fish & Wildlife Service; US Fish & Wildlife Service Bioko Biodiversity Protection Program CHE-0958973 / NSF Major Research Instrumentation; National Science Foundation (NSF) Drexel University ExxonMobil Foundation; Exxon Mobil Corporation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000335224900015
- Scopus ID
- 2-s2.0-84899536619
- Other Identifier
- 991019169666004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Analytical
- Food Science & Technology
- Spectroscopy