Journal article
Nonthermal Dielectric-Barrier Discharge Plasma-Induced Inactivation Involves Oxidative DNA Damage and Membrane Lipid Peroxidation in Escherichia coli
Antimicrobial agents and chemotherapy, v 55(3), pp 1053-1062
Mar 2011
PMID: 21199923
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Oxidative stress leads to membrane lipid peroxidation, which yields products causing variable degrees of detrimental oxidative modifications in cells. Reactive oxygen species (ROS) are the key regulators in this process and induce lipid peroxidation in
Escherichia coli
. Application of nonthermal (cold) plasma is increasingly used for inactivation of surface contaminants. Recently, we reported a successful application of nonthermal plasma, using a floating-electrode dielectric-barrier discharge (FE-DBD) technique for rapid inactivation of bacterial contaminants in normal atmospheric air (S. G. Joshi et al., Am. J. Infect. Control 38:293-301, 2010). In the present report, we demonstrate that FE-DBD plasma-mediated inactivation involves membrane lipid peroxidation in
E. coli
. Dose-dependent ROS, such as singlet oxygen and hydrogen peroxide-like species generated during plasma-induced oxidative stress, were responsible for membrane lipid peroxidation, and ROS scavengers, such as α-tocopherol (vitamin E), were able to significantly inhibit the extent of lipid peroxidation and oxidative DNA damage. These findings indicate that this is a major mechanism involved in FE-DBD plasma-mediated inactivation of bacteria.
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Details
- Title
- Nonthermal Dielectric-Barrier Discharge Plasma-Induced Inactivation Involves Oxidative DNA Damage and Membrane Lipid Peroxidation in Escherichia coli
- Creators
- Suresh G Joshi - Surgical Infection Program, Department of Surgery, and Department of Microbiology and Immunology, Drexel University College of MedicineMoogega Cooper - Surgical Infection Program, Department of Surgery, and Department of Microbiology and Immunology, Drexel University College of MedicineAdam Yost - Surgical Infection Program, Department of Surgery, and Department of Microbiology and Immunology, Drexel University College of MedicineMichelle Paff - Surgical Infection Program, Department of Surgery, and Department of Microbiology and Immunology, Drexel University College of MedicineUtku K Ercan - Surgical Infection Program, Department of Surgery, and Department of Microbiology and Immunology, Drexel University College of MedicineGregory Fridman - Surgical Infection Program, Department of Surgery, and Department of Microbiology and Immunology, Drexel University College of MedicineGary Friedman - Surgical Infection Program, Department of Surgery, and Department of Microbiology and Immunology, Drexel University College of MedicineAlexander Fridman - Surgical Infection Program, Department of Surgery, and Department of Microbiology and Immunology, Drexel University College of MedicineAri D Brooks - Surgical Infection Program, Department of Surgery, and Department of Microbiology and Immunology, Drexel University College of Medicine
- Publication Details
- Antimicrobial agents and chemotherapy, v 55(3), pp 1053-1062
- Publisher
- American Society for Microbiology (ASM)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Mechanical Engineering and Mechanics; Surgery
- Web of Science ID
- WOS:000287687100014
- Scopus ID
- 2-s2.0-79952337526
- Other Identifier
- 991014877767204721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Microbiology
- Pharmacology & Pharmacy