Journal article
Norepinephrine stimulates arachidonic acid release from vascular smooth muscle via activation of cPLA 2
American Journal of Physiology: Cell Physiology, v 274(4), pp C1129-C1137
01 Apr 1998
PMID: 29585807
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The mechanism of agonist-activated arachidonate release was studied in segments of rat tail artery. Tail artery segments were prelabeled with [
3
H]arachidonate and then stimulated with norepinephrine (NE), and the radioactivity of the extracellular medium was determined. NE stimulated arachidonate release from the tissue without increasing arachidonic acid levels within cellular cytosol or crude membranes. About 90% of the extracellular radioactivity was shown to be unmetabolized arachidonate by TLC. Arachidonic acid release was not inhibited by the removal of the endothelium from the artery. NE exerted a half-maximal effect at a concentration of 0.2 μM. NE-stimulated arachidonate release was not inhibited by blockers of phospholipase C (U-73122), diacylglycerol lipase (RHC-80267), secretory phospholipase A
2
(manoalide), calcium-insensitive phospholipase A
2
(HELSS), or β-adrenergic receptors (propranolol). NE-stimulated arachidonic acid release was inhibited by blockers of cytosolic phospholipase A
2
(cPLA
2
) (AACOCF
3
), α
1
-adrenergic receptors (prazosin), and specific G proteins (pertussis toxin). This indicated that NE stimulated arachidonate release from vascular smooth muscle via activation of α-adrenergic receptors, either G
i
or G
o
, and cPLA
2
. NE-activated arachidonic acid release from vascular smooth muscle may play a role in force generation by the tissue. Perhaps arachidonic acid extends the effect of NE on one specific smooth muscle cell to its nearby neighbor cells.
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Details
- Title
- Norepinephrine stimulates arachidonic acid release from vascular smooth muscle via activation of cPLA 2
- Creators
- Edward F. LaBelle - Allegheny University of the Health SciencesErzsebet Polyak - Allegheny University of the Health SciencesEmil Polyak - Digital Media
- Publication Details
- American Journal of Physiology: Cell Physiology, v 274(4), pp C1129-C1137
- Publisher
- American Physiological Society (APS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Digital Media
- Web of Science ID
- WOS:000072850000033
- Scopus ID
- 2-s2.0-0031957001
- Other Identifier
- 991019168352204721
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- Web of Science research areas
- Cell Biology
- Physiology