Journal article
Normobaric Oxygen (NBO) Therapy Reduces Cerebral Ischemia/Reperfusion Injury through Inhibition of Early Autophagy
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, v 2021, 7041290
30 Jun 2021
PMID: 34306153
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Objectives. Normobaric oxygen (NBO) therapy has great clinical potential in the treatment of ischemic stroke, but its underlying mechanism is unknown. Our study aimed to investigate the role of autophagy during the application of NBO on cerebral ischemia/reperfusion injury. Methods. Male Sprague Dawley rats received 2 hours of middle cerebral artery occlusion (MCAO), followed by 2, 6, or 24 hours of reperfusion. At the beginning of reperfusion, rats were randomly given NBO (95% O-2) or room air (21% O-2) for 2 hours. In some animals, 3-methyladenine (3-MA, autophagy inhibitor) was administered 10 minutes before reperfusion. The severity of the ischemic injury was determined by infarct volume, neurological deficit, and apoptotic cell death. Western blotting was used to determine the protein expression of autophagy and apoptosis, while mRNA expression of apoptotic molecules was detected by real-time PCR. Results. NBO treatment after ischemia/reperfusion significantly decreased infarct volume and neurobehavioral defects. The increased expression of the autophagy markers, including microtubule-associated protein 1A light chain 3 (LC3) and Beclin 1, after ischemia/reperfusion was reversed by NBO, while promoting Sequestosome 1 (p62/SQSTM1) expression. In addition, NBO reduced cerebral apoptosis in association with alleviated BAX expression and increased BCL-2 expression. 3-MA reduced autophagy and apoptotic death but did not further improve NBO-attenuated ischemic damage. Conclusion. NBO induced remarkable neuroprotection from ischemic injury, which was correlated with blocked autophagy activity.
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Details
- Title
- Normobaric Oxygen (NBO) Therapy Reduces Cerebral Ischemia/Reperfusion Injury through Inhibition of Early Autophagy
- Publication Details
- EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, v 2021, 7041290
- Publisher
- HINDAWI LTD; LONDON
- Grant note
- This work was partially supported by the National Natural Science Foundation of China (81871838 and 82072549), the Science and Technology Plan of Beijing Tongzhou District (KJ2020CX002), and the Natural Science Foundation of Beijing Municipality (7214239).
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Drexel University
- Web of Science ID
- WOS:000674605400003
- Scopus ID
- 2-s2.0-85110142054
- Other Identifier
- 991021860662204721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Integrative & Complementary Medicine