Journal article
Novel Production of Terpenoids in Escherichia coli and Activities Against Breast Cancer Cell Lines
Applied biochemistry and biotechnology, v 175(5), pp 2319-2331
01 Mar 2015
PMID: 25484192
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Metabolic engineering of heterologous pathways has allowed the production of therapeutically important compounds in microbial systems. Here, we report the engineering of a monoterpenoid biosynthetic pathway into Escherichia coli. Five genes encoding sequential enzymes for perillyl alcohol biosynthesis from the precursors isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) were engineered into E. coli. Expression of these genes allowed the production of the intermediate limonene, but the downstream monoterpenoid, perillyl alcohol, was not detected. A new compound was detected but could not be identified based on the data obtained. Only 1.6 mu g/ml of the compound was being produced from the engineered E. coli strain, but, when these cultures were fed limonene as a substrate, the production was nearly 250 mu g/ml. This unknown compound inhibited the cell proliferation of MCF-7 and MDA-MB-231 breast cancer cells in 48-h treatment experiments. This compound may have potential benefits in breast cancer treatment. This is the first report showing the production of a monoterpenoid in engineered E. coli and its antiproliferative effects in breast cancer cells.
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Details
- Title
- Novel Production of Terpenoids in Escherichia coli and Activities Against Breast Cancer Cell Lines
- Creators
- Shweta Gupta - University of MichiganMelissa G. Marko - University of the SciencesVandana A. Miller - Drexel UniversityFrederick T. Schaefer - University of the SciencesJennifer R. Anthony - Delaware County Community CollegeJohn R. Porter - University of the Sciences
- Publication Details
- Applied biochemistry and biotechnology, v 175(5), pp 2319-2331
- Publisher
- Springer Nature
- Number of pages
- 13
- Grant note
- College of Graduate Studies, University of the Sciences Department of Biological Sciences, University of the Sciences
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; Microbiology Department Internal Research
- Web of Science ID
- WOS:000350879200001
- Scopus ID
- 2-s2.0-84924033634
- Other Identifier
- 991021448051504721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biotechnology & Applied Microbiology