Journal article
Novel Tick-Borne Anaplasmataceae Genotypes in Tropical Birds from the Brazilian Pantanal Wetland
Microorganisms (Basel), v 12(5), 962
01 May 2024
PMID: 38792791
Abstract
Despite numerous reports of Anaplasmataceae agents in mammals worldwide, few studies have investigated their occurrence in birds. The present study aimed to investigate the occurrence and molecular identity of Anaplasmataceae agents in birds from the Pantanal wetland, Brazil. Blood samples were collected from 93 different species. After DNA extraction, samples positive for the avian beta-actin gene were subjected to both a multiplex quantitative real-time (q)PCR for Anaplasma and Ehrlichia targeting the groEL gene and to a conventional PCR for Anaplasmataceae agents targeting the 16S rRNA gene. As a result, 37 (7.4%) birds were positive for Anaplasma spp. and 4 (0.8%) for Ehrlichia spp. in the qPCR assay; additionally, 13 (2.6%) were positive for Anaplasmataceae agents in the PCR targeting the 16S rRNA gene. The Ehrlichia 16S rRNA sequences detected in Arundinicola leucocephala, Ramphocelus carbo, and Elaenia albiceps were positioned closely to Ehrlichia sp. Magellanica. Ehrlichia dsb sequences detected in Agelasticus cyanopus and Basileuterus flaveolus grouped with Ehrlichia minasensis. The 16S rRNA genotypes detected in Crax fasciolata, Pitangus sulphuratus and Furnarius leucopus grouped with Candidatus Allocryptoplasma. The 23S-5S genotypes detected in C. fasciolata, Basileuterus flaveolus, and Saltator coerulescens were related to Anaplasma phagocytophilum. In conclusion, novel genotypes of Anaplasma, Ehrlichia, and Candidatus Allocryptoplasma were detected in birds from the Pantanal wetland.
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Details
- Title
- Novel Tick-Borne Anaplasmataceae Genotypes in Tropical Birds from the Brazilian Pantanal Wetland
- Creators
- Amir Salvador Alabi Cordova - Universidade Estadual Paulista (Unesp)Alan Fecchio - Drexel UniversityAna Claudia Calchi - Universidade Estadual Paulista (Unesp)Clara Morato Dias - Universidade Estadual Paulista (Unesp)Anna Claudia Baumel Mongruel - Universidade Estadual Paulista (Unesp)Lorena Freitas das Neves - Universidade Estadual Paulista (Unesp)Daniel Antonio Braga Lee - Universidade Estadual Paulista (Unesp)Rosangela Zacarias Machado - Universidade Estadual Paulista (Unesp)Marcos Rogerio Andre - Universidade Estadual Paulista (Unesp)
- Publication Details
- Microorganisms (Basel), v 12(5), 962
- Publisher
- MDPI
- Number of pages
- 15
- Grant note
- Fundao de Amparo Pesquisa do Estado de So Paulo (FAPESP); Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Ornithology
- Web of Science ID
- WOS:001231516900001
- Scopus ID
- 2-s2.0-85194261314
- Other Identifier
- 991022202123904721