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Journal article
Novel Tools for Genetic Manipulation of Follicle Stem Cells in the Drosophila Ovary Reveal an Integrin-Dependent Transition from Quiescence to Proliferation
Genetics (Austin), v 199(4), pp 935-957
12 Feb 2015
PMID: 25680813
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In many tissues, the presence of stem cells is inferred by the capacity of the tissue to maintain homeostasis and undergo repair after injury. Isolation of self-renewing cells with the ability to generate the full array of cells within a given tissue strongly supports this idea, but the identification and genetic manipulation of individual stem cells within their niche remain a challenge. Here we present novel methods for marking and genetically altering epithelial follicle stem cells (FSCs) within the
Drosophila
ovary. Using these new tools, we define a sequential multistep process that comprises transitioning of FSCs from quiescence to proliferation. We further demonstrate that integrins are cell-autonomously required within FSCs to provide directional signals that are necessary at each step of this process. These methods may be used to define precise roles for specific genes in the sequential events that occur during FSC division after a period of quiescence.
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Details
- Title
- Novel Tools for Genetic Manipulation of Follicle Stem Cells in the Drosophila Ovary Reveal an Integrin-Dependent Transition from Quiescence to Proliferation
- Creators
- Tiffiney R. Hartman - Fox Chase Cancer CenterErin M. Ventresca - Fox Chase Cancer CenterAnthony Hopkins - Fox Chase Cancer CenterDaniel Zinshteyn - Fox Chase Cancer CenterTanu Singh - Fox Chase Cancer CenterJenny A. O’Brien - Fox Chase Cancer CenterBenjamin C. Neubert - Fox Chase Cancer CenterMatthew G. Hartman - Fox Chase Cancer CenterHeather K. Schofield - Fox Chase Cancer CenterKevin P. Stavrides - Fox Chase Cancer CenterDanielle E. Talbot - Fox Chase Cancer CenterDevon J. Riggs - Fox Chase Cancer CenterCaroline Pritchard - Fox Chase Cancer CenterAlana M. O’Reilly - Fox Chase Cancer Center
- Publication Details
- Genetics (Austin), v 199(4), pp 935-957
- Publisher
- Genetics Society of America
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Radiology (Radiologic Sciences); School of Education
- Web of Science ID
- WOS:000352734400005
- Scopus ID
- 2-s2.0-84926513012
- Other Identifier
- 991019168383304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Genetics & Heredity