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Novel Tools for Genetic Manipulation of Follicle Stem Cells in the Drosophila Ovary Reveal an Integrin-Dependent Transition from Quiescence to Proliferation
Journal article   Open access   Peer reviewed

Novel Tools for Genetic Manipulation of Follicle Stem Cells in the Drosophila Ovary Reveal an Integrin-Dependent Transition from Quiescence to Proliferation

Tiffiney R. Hartman, Erin M. Ventresca, Anthony Hopkins, Daniel Zinshteyn, Tanu Singh, Jenny A. O’Brien, Benjamin C. Neubert, Matthew G. Hartman, Heather K. Schofield, Kevin P. Stavrides, …
Genetics (Austin), v 199(4), pp 935-957
12 Feb 2015
PMID: 25680813
url
https://doi.org/10.1534/genetics.114.173617View
Published, Version of Record (VoR)Open Access (License Unspecified) Open

Abstract

adhesion follicle stem cell germarium integrin Investigations quiescence
In many tissues, the presence of stem cells is inferred by the capacity of the tissue to maintain homeostasis and undergo repair after injury. Isolation of self-renewing cells with the ability to generate the full array of cells within a given tissue strongly supports this idea, but the identification and genetic manipulation of individual stem cells within their niche remain a challenge. Here we present novel methods for marking and genetically altering epithelial follicle stem cells (FSCs) within the Drosophila ovary. Using these new tools, we define a sequential multistep process that comprises transitioning of FSCs from quiescence to proliferation. We further demonstrate that integrins are cell-autonomously required within FSCs to provide directional signals that are necessary at each step of this process. These methods may be used to define precise roles for specific genes in the sequential events that occur during FSC division after a period of quiescence.

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Collaboration types
Domestic collaboration
Web of Science research areas
Genetics & Heredity
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