Journal article
Nuclemeter: A Reaction-Diffusion Based Method for Quantifying Nucleic Acids Undergoing Enzymatic Amplification
Scientific reports, v 4(1), pp 7335-7335
05 Dec 2014
PMID: 25477046
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Real-time amplification and quantification of specific nucleic acid sequences plays a major role in medical and biotechnological applications. In the case of infectious diseases, such as HIV, quantification of the pathogen-load in patient specimens is critical to assess disease progression and effectiveness of drug therapy. Typically, nucleic acid quantification requires expensive instruments, such as real-time PCR machines, which are not appropriate for on-site use and for low-resource settings. This paper describes a simple, low-cost, reaction-diffusion based method for end-point quantification of target nucleic acids undergoing enzymatic amplification. The number of target molecules is inferred from the position of the reaction-diffusion front, analogous to reading temperature in a mercury thermometer. The method was tested for HIV viral load monitoring and performed on par with conventional benchtop methods. The proposed method is suitable for nucleic acid quantification at point of care, compatible with multiplexing and high-throughput processing, and can function instrument-free.
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Details
- Title
- Nuclemeter: A Reaction-Diffusion Based Method for Quantifying Nucleic Acids Undergoing Enzymatic Amplification
- Creators
- Changchun Liu - University of PennsylvaniaMohamed M. Sadik - University of PennsylvaniaMichael G. Mauk - Univ Penn, Sch Engn & Appl Sci, Dept Mech Engn & Appl Mech, Philadelphia, PA 19104 USAPaul H. Edelstein - University of PennsylvaniaFrederic D. Bushman - University of PennsylvaniaRobert Gross - University of PennsylvaniaHaim H. Bau - University of Pennsylvania
- Publication Details
- Scientific reports, v 4(1), pp 7335-7335
- Publisher
- Springer Nature
- Number of pages
- 7
- Grant note
- Nanotechnology Institute of the Pennsylvania Department of Community and Economic Development Ben Franklin Technology Development Authority K25AI099160 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) K25AI099160; 1R41AI104418-01A1 / NIH/NIAID; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) AI045008 / Penn Center for AIDS Research (CFAR) Pilot Grant
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Engineering Technology
- Web of Science ID
- WOS:000346270100006
- Scopus ID
- 2-s2.0-84923283457
- Other Identifier
- 991020623759404721
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