Logo image
Back
Nutraceutical Apigenin regulates DC function in a RelB-dependent manner during neuroinflammation
Journal article   Peer reviewed

Nutraceutical Apigenin regulates DC function in a RelB-dependent manner during neuroinflammation

Pooja Jain, Rashida Ginwala, Emily McTish, Patrick Moore, Nikil Revuri, Chander Raman, Narendra Singh, Mitzi Nagarkatti, Prakash Nagarkatti, Victoria A Kranz, …
The Journal of immunology (1950), v 208(1_Supplement)
01 May 2022
DOI: https://doi.org/10.4049/jimmunol.208.Supp.162.02

Abstract

Abstract Apigenin, a natural flavonoid, found in several plants is known to have anti-oxidant and anti-inflammatory properties indicated by its use for centuries as a medicinal approach to treat inflammatory disorders. However, there are significant gaps in knowledge regarding its effect on dendritic cell (DC) function in maintaining an immune balance in immunospecialized locations like the central nervous system (CNS). In order to establish the potential utility of Apigenin as a therapeutic agent against neuroinflammatory diseases, we tested and found that Apigenin treatment ameliorated severity of disease progression and relapse after onset of experimental autoimmune encephalomyelitis (EAE) in C57BL/6 and SJL mouse models of multiple sclerosis. An increased retention of DCs and other myeloid cells in the periphery correlated with decreased immune cell infiltration and reduced demyelination in the CNS in treated mice. Mechanistically, Apigenin treatment reduced RelB expression in presence of LPS in human peripheral blood DCs, which is central to DC maturation, its antigen presentation capabilities and DC-mediated T cell activation. IL-12A and IL-23, downstream pro-inflammatory targets of RelB were reduced upon Apigenin treatment in these cells. Further, RelB causes a metabolic switch in immune cells upon inflammation, which was seen as a decrease in glucose uptake and lactate production (glycolysis), and an increase in mitochondrial activity when LPS-induced DCs were treated with Apigenin. These results indicate a protective role of Apigenin against DC-regulated neurodegenerative effects through a probable RelB mediated pathway thus implicating a potential therapy for neuroinflammatory disease. Supported by grants from NIH: 1R01NS0971-47

Metrics

26 Record Views

Details

InCites Highlights

Data related to this publication, from InCites Benchmarking & Analytics tool:

Web of Science research areas
Immunology
Logo image