Journal article
Odorant-receptor-mediated regulation of chemosensory gene expression in the malaria mosquito Anopheles gambiae
Cell reports (Cambridge), v 38(10), pp 110494-110494
08 Mar 2022
PMID: 35263579
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Mosquitoes locate and approach humans based on the activity of odorant receptors (ORs) expressed on olfactory receptor neurons (ORNs). Olfactogenetic experiments in Anopheles gambiae mosquitoes revealed that the ectopic expression of an AgOR (AgOR2) in ORNs dampened the activity of the expressing neuron. This contrasts with studies in Drosophila melanogaster in which the ectopic expression of non-native ORs in ORNs confers ectopic neuronal responses without interfering with native olfactory physiology. RNA-seq analyses comparing wild-type antennae to those ectopically expressing AgOR2 in ORNs indicated that nearly all AgOR transcripts were significantly downregulated (except for AgOR2). Additional experiments suggest that AgOR2 protein rather than mRNA mediates this downregulation. Using in situ hybridization, we find that AgOR gene choice is active into adulthood and that AgOR2 expression inhibits AgORs from turning on at this late stage. Our study shows that the ORNs of Anopheles mosquitoes (in contrast to Drosophila) are sensitive to a currently unexplored mechanism of AgOR regulation.
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Details
- Title
- Odorant-receptor-mediated regulation of chemosensory gene expression in the malaria mosquito Anopheles gambiae
- Creators
- Sarah E. Maguire - Johns Hopkins MedicineAli Afify - Johns Hopkins MedicineLoyal A. Goff - Johns Hopkins MedicineChristopher J. Potter - Johns Hopkins Medicine
- Publication Details
- Cell reports (Cambridge), v 38(10), pp 110494-110494
- Publisher
- Elsevier
- Number of pages
- 19
- Grant note
- W81XWH-17-PRMRP / Department of Defense; United States Department of Defense Johns Hopkins Malaria Research Institute postdoctoral fellowship Bloomberg Philanthropies NIAID R01Al137078 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Johns Hopkins Malaria Research Institute
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000768326700006
- Scopus ID
- 2-s2.0-85126076232
- Other Identifier
- 991021229904104721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Cell Biology