Journal article
Oligoclonal T cell expansion in the skin of patients with systemic sclerosis
The Journal of immunology (1950), Vol.168(7), pp.3649-3659
01 Apr 2002
PMID: 11907131
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Fibrosis, microvascular fibroproliferative alterations, and autoantibody production are the main features of systemic sclerosis (SSc), and all of them can be explained by cytokine production by activated T cells. However, little is known about the role of T cells in the pathogenesis of SSc, and there is no information on the Ag(s) that elicits such activation. To determine whether T cells infiltrating the skin biopsies of patients with SSc are oligoclonal, beta-chain TCR transcripts from T cells infiltrating the skin of five patients with SSc of recent onset were amplified by either Vbeta-specific PCR or nonpalindromic adaptor PCR. The resulting PCR products were subsequently cloned and sequenced. High proportions of identical beta-chain TCR transcripts ranging from 43 to 90% of those sequenced were found in five patients, strongly suggesting the presence of oligoclonal T cells in these infiltrates. A dominant T cell clone was found to be clonally expanded in skin biopsies obtained from a single patient with SSc at three different times (0, 8, and 13 mo earlier) and from three different skin regions. beta-chain TCR transcripts from PBMC from normal donors (methodological control) were unique when compared with each other, typical for polyclonal populations of T cells. The finding of oligoclonal T cells infiltrating the skin of patients with SSc suggests that these T cells have undergone proliferation in situ in the skin and clonal expansion in response to as yet unidentified Ag(s). These results suggest that T cells are involved in the pathogenesis of the disease.
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Details
- Title
- Oligoclonal T cell expansion in the skin of patients with systemic sclerosis
- Creators
- Lazaros I Sakkas - Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140, USABin XuCarol M ArtlettSong LuSergio A JimenezChris D Platsoucas
- Publication Details
- The Journal of immunology (1950), Vol.168(7), pp.3649-3659
- Publisher
- American Association of Immunologists (AAI); United States
- Grant note
- R01 AR48042 / NIAMS NIH HHS T32AI07101 / NIAID NIH HHS R01 AM19616 / NIADDK NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Identifiers
- 991014878175604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Immunology