Journal article
Ontogenesis of the murine hepatic extracellular matrix: an immunohistochemical study
Differentiation (London), v 51(3), pp 209-218
1992
PMID: 1459362
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
To define the role of the extracellular matrix (ECM) in hepatogenesis, we examined the temporal and spatial deposition of fibronectin, laminin and collagen types I and IV in 12.5–21.5 day fetal and 1, 7 and 14 day postnatal rat livers. In early fetal liver, discontinuous deposits of the four ECM components studied were present in the perisinusoidal space, with laminin being the most prevalent. All basement membrane zones contained collagen type IV and laminin, including those of the capsule (mesothelial), portal vein radicles and bile ductules. Fibronectin had a distribution similar to that of collagen type IV early in gestation. However, at later gestational dates, fibronectin distribution in the portal triads approached that of collagen type I, being present in the interstitial connective tissues; whereas, collagen type IV and laminin were restricted to vascular and biliary basement membrane zones in those regions.
The cytoplasm of some sinusoidal lining cells and hepatocytes reacted with antibodies to extracellular matrix components. By electron microscopy the immunoreactive material was localized in the endoplasmic reticulum, indicating the ability of these cells to synthesize these ECM proteins. Biliary ductular cells had prominent intracytoplasmic staining for laminin and collagen type IV from day 19.5 gestation until 7 days of postnatal life, but lacked demonstrable fibronectin or collagen type I.
These results demonstrate that by 12.5 days of gestation the rat liver anlage has deposited a complex extracellular matrix in the perisinusoidal space. The prevalence of laminin in the developing hepatic lobules suggests a possible role for this glycoprotein in hepatic morphogenesis. In view of the intimate association of the hepatic lobular extracellular matrix with the developing vasculature, we hypothesize that laminin provides a scaffold of the developing liver, but once the ontogenesis is complete, intrahepatic perisinusoidal laminin expression is suppressed.
Metrics
Details
- Title
- Ontogenesis of the murine hepatic extracellular matrix: an immunohistochemical study
- Creators
- Zubair Baloch - Hahnemann University HospitalJoseph Klapper - Hahnemann University HospitalLinda Buchanan - Hahnemann University HospitalMary Schwartz - Hahnemann University HospitalPeter Sebastian Amenta - University of Medicine and Dentistry of New JerseyMalcolm S Schwartz - Pediatrics
- Publication Details
- Differentiation (London), v 51(3), pp 209-218
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics
- Web of Science ID
- WOS:A1992JV63600008
- Scopus ID
- 2-s2.0-0026660668
- Other Identifier
- 991019184026004721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology
- Developmental Biology