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Journal article
Opiate agonist-induced re-distribution of Wntless, a mu-opioid receptor interacting protein, in rat striatal neurons
Experimental neurology, v 233(1), pp 205-213
01 Jan 2012
PMID: 22001156
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Wntless (WLS), a mu-opioid receptor (MOR) interacting protein, mediates Wnt protein secretion that is critical for neuronal development. We investigated whether MOR agonists induce re-distribution of WLS within rat striatal neurons. Adult male rats received either saline, morphine or [D-Ala2, N-Me-Phe4, Gly-ol5]-enkephalin (DAMGO) directly into the lateral ventricles. Following thirty minutes, brains were extracted and tissue sections were processed for immunogold silver detection of WLS. In saline-treated rats, WLS was distributed along the plasma membrane and within the cytoplasmic compartment of striatal dendrites as previously described. The ratio of cytoplasmic to total dendritic WLS labeling was 0.70 +/- 0.03 in saline-treated striatal tissue. Morphine treatment decreased this ratio to 0.48 +/- 0.03 indicating a shift of WLS from the intracellular compartment to the plasma membrane. However, following DAMGO treatment, the ratio was 0.85 +/- 0.05 indicating a greater distribution of WLS intracellularly. The difference in the re-distribution of the WLS following different agonist exposure may be related to DAMGO's well known ability to induce internalization of MOR in contrast to morphine, which is less effective in producing receptor internalization. Furthermore, these data are consistent with our hypothesis that MOR agonists promote dimerization of WLS and MOR, thereby preventing WLS from mediating Wnt secretion. In summary, our findings indicate differential agonist-induced trafficking of WLS in striatal neurons following distinct agonist exposure. Adaptations in WLS trafficking may represent a novel pharmacological target in the treatment of opiate addiction and/or pain. (C) 2011 Elsevier Inc. All rights reserved.
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Details
- Title
- Opiate agonist-induced re-distribution of Wntless, a mu-opioid receptor interacting protein, in rat striatal neurons
- Creators
- B. A. S. Reyes - Jefferson Hospital for NeuroscienceK. Vakharia - Thomas Jefferson UniversityT. N. Ferraro - College Station Medical CenterR. Levenson - Pennsylvania State UniversityW. H. Berrettini - College Station Medical CenterE. J. Van Bockstaele - Thomas Jefferson University
- Publication Details
- Experimental neurology, v 233(1), pp 205-213
- Publisher
- Elsevier
- Number of pages
- 9
- Grant note
- P20DA025995 / NATIONAL INSTITUTE ON DRUG ABUSE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Drug Abuse (NIDA); European Commission P20 DA 025995; DA 05186; DA 09082 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000300123900024
- Scopus ID
- 2-s2.0-84856229104
- Other Identifier
- 991021903411104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences