Journal article
Opposite Effect of Protein Synthesis Inhibitors on Potassium Deficiency‐Induced Apoptotic Cell Death in Immature and Mature Neuronal Cultures
Journal of neurochemistry, v 65(3), pp 1395-1398
Sep 1995
PMID: 7643118
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Abstract
: Typically, primary cultures of rat cerebellar granule neurons are grown in the presence of 25 mM KCl and are considered to mature by ∼7 days in vitro. Potassium deficiency was created by growing the neurons from days 1 to 4 in the presence of 12.5 mM KCl (immature cultures) or by switching the mature neurons grown with 25 mM KCl to 12.5 mM KCl. In both conditions we observed neuronal death that bears the signs of apoptosis, i.e., DNA fragmentation determined qualitatively by agarose gel electrophoresis of DNA and quantitatively by in situ terminal deoxynucleotidyl transferase assay. The protein synthesis inhibitors cycloheximide and anisomycin provided neuroprotection in the mature cultures but potentiated the toxic effect of KCl deprivation in the immature neurons. The results suggest that a prudent use of protein synthesis inhibitors is critical in experiments with primary neuronal cultures.
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Details
- Title
- Opposite Effect of Protein Synthesis Inhibitors on Potassium Deficiency‐Induced Apoptotic Cell Death in Immature and Mature Neuronal Cultures
- Creators
- Elena Kharlamov - Drexel UniversityCinzia M. Cagnoli - Drexel UniversityCagla Atabay - Drexel UniversitySnežana Ikonomović - Drexel UniversityDennis R. Grayson - Drexel UniversityHari Manev - Drexel University
- Publication Details
- Journal of neurochemistry, v 65(3), pp 1395-1398
- Publisher
- Blackwell Science Ltd
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurology
- Web of Science ID
- WOS:A1995RQ57100054
- Scopus ID
- 2-s2.0-0029132805
- Other Identifier
- 991019184035104721
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- Web of Science research areas
- Biochemistry & Molecular Biology
- Neurosciences