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Optimization of Non-Thermal Plasma Treatment in an In Vivo Model Organism
Journal article   Open access   Peer reviewed

Optimization of Non-Thermal Plasma Treatment in an In Vivo Model Organism

Amanda Lee, Abraham Lin, Kajol Shah, Harpreet Singh, Vandana Miller, Shubha Gururaja Rao and Alice Lee
PloS one, v 11(8), pp e0160676-e0160676
2016
DOI: https://doi.org/10.1371/journal.pone.0160676
PMID: 27505063
Featured in Collection :   UN Sustainable Development Goals @ Drexel
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https://doi.org/10.1371/journal.pone.0160676View
Published, Version of Record (VoR)CC BY V4.0,  Open

Abstract

Animals Blood Cells - cytology Blood Cells - drug effects Cell Differentiation - drug effects Dose-Response Relationship, Drug Drosophila melanogaster - cytology Drosophila melanogaster - drug effects Drosophila melanogaster - metabolism Drosophila melanogaster - physiology Fertility - drug effects Larva - drug effects Larva - physiology Locomotion - drug effects Lymph Nodes - drug effects Lymph Nodes - metabolism Plasma Gases - pharmacology Reactive Oxygen Species - metabolism Temperature
Non-thermal plasma is increasingly being recognized for a wide range of medical and biological applications. However, the effect of non-thermal plasma on physiological functions is not well characterized in in vivo model systems. Here we use a genetically amenable, widely used model system, Drosophila melanogaster, to develop an in vivo system, and investigate the role of non-thermal plasma in blood cell differentiation. Although the blood system in Drosophila is primitive, it is an efficient system with three types of hemocytes, functioning during different developmental stages and environmental stimuli. Blood cell differentiation in Drosophila plays an essential role in tissue modeling during embryogenesis, morphogenesis and also in innate immunity. In this study, we optimized distance and frequency for a direct non-thermal plasma application, and standardized doses to treat larvae and adult flies so that there is no effect on the viability, fertility or locomotion of the organism. We discovered that at optimal distance, time and frequency, application of plasma induced blood cell differentiation in the Drosophila larval lymph gland. We articulate that the augmented differentiation could be due to an increase in the levels of reactive oxygen species (ROS) upon non-thermal plasma application. Our studies open avenues to use Drosophila as a model system in plasma medicine to study various genetic disorders and biological processes where non-thermal plasma has a possible therapeutic application.

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7 citations in Web of Science
9 citations in Scopus

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Web of Science research areas
Physics, Applied
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