Journal article
Optimization of antibody immobilization for sensing using piezoelectrically excited-millimeter-sized cantilever (PEMC) sensors
Sensors and actuators. B, Chemical, v 123(1), pp 474-479
2007
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The effectiveness of antibody immobilization on sensor performance was evaluated using a piezoelectric-excited millimeter-sized cantilever (PEMC) sensor and a model protein, bovine serum albumin (BSA). The immobilization parameters—antibody and activation reagent concentration and reaction time were systematically varied and the resultant sensor response to 1
pg/mL BSA was measured.
The highest frequency shift due to BSA attachment (1931
±
60
Hz) was obtained when 100
μg/mL antibody solution was activated with EDC (2
mM) and sulfo-NHS (5
mM) for 30
min prior to reaction with primary amine on the sensor surface. Increasing activation time from 30 to 60
min resulted in an 8% decrease in sensor response, while activation at 0.2
mM EDC and 0.5
mM NHS resulted in a 65% decrease. The logarithmic correlation between antibody concentration and sensor response suggests that a lower antibody concentration of 10
μg/mL is sufficient for BSA at 1
pg/mL. With 10
μg/mL antibody, the PEMC sensor response was 725
±
50
Hz (
n
=
2) for 1
pg/mL BSA with a signal to noise ratio of 41.
Metrics
Details
- Title
- Optimization of antibody immobilization for sensing using piezoelectrically excited-millimeter-sized cantilever (PEMC) sensors
- Creators
- David MaraldoRaj Mutharasan
- Publication Details
- Sensors and actuators. B, Chemical, v 123(1), pp 474-479
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000246171200069
- Scopus ID
- 2-s2.0-34047153888
- Other Identifier
- 991014878288904721
UN Sustainable Development Goals (SDGs)
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Analytical
- Electrochemistry
- Instruments & Instrumentation