Journal article
Optimization of arylacetonitrilase production from Alcaligenes sp MTCC 10675 and its application in mandelic acid synthesis
Applied microbiology and biotechnology, v 98(1)
01 Jan 2014
PMID: 24104468
Abstract
Alcaligenes sp. MTCC 10675 has been isolated from soil sample using enrichment method and has nitrilase catalytic system which is highly specific for the hydrolysis of arylaliphatic nitriles. Optimization of culture conditions using response surface methodology and inducer-mediated approach enhanced arylacetonitrilase production significantly (2.4-fold). Isobutyronitrile acted as an effective inducer for the induction of arylacetonitrilase, and it is highly specific for arylacetonitriles (phenyl acetonitrile and mandelonitrile). Arylacetonitrilase has no effect on its relative velocity (V (r)) up to 20 mM substrate (mandelonitrile) concentration and at 30 mM mandelonitrile, 23.4 % degree of inhibition (I (d)) was recorded. Half life of arylacetonitrilase of Alcaligenes sp. MTCC 10675 was 27.5 h at 25 A degrees C. Hg2+, Ag+, Pb3+, and Co2+ were strong inhibitor of arylacetonitrilase activity which resulted into 100 %, 91 %, 84 %, and 83 % inhibition, respectively. Polar protic solvent (dichloromethane, dimethylsulphooxide, and n-butanol) reduce arylacetonitrilase activity up to 80-94 % at 10 % concentration. Alcaligenes sp. MTCC 10675 has higher biocatalytic activity, i.e., 3.9 gg(-1) dcw, which is highest in comparison to till reported organism. Arylacetonitrilase-mediated hydrolysis of racemic mandelonitrile resulted into R-(-) mandelic acid with 99.0 % enantiomeric excess (e.e.).
Metrics
Details
- Title
- Optimization of arylacetonitrilase production from Alcaligenes sp MTCC 10675 and its application in mandelic acid synthesis
- Creators
- S. K. Bhatia - Himachal Pradesh UniversityP. K. Mehta - Himachal Pradesh UniversityR. K. Bhatia - Himachal Pradesh UniversityT. C. Bhalla - Himachal Pradesh University
- Publication Details
- Applied microbiology and biotechnology, v 98(1)
- Publisher
- Springer Nature
- Number of pages
- 12
- Grant note
- Department of Biotechnology University Grant Commission, India; University Grants Commission, India
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000329093600008
- Scopus ID
- 2-s2.0-84891875741
- Other Identifier
- 991021229907304721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biotechnology & Applied Microbiology