Journal article
Optimization of matrix-assisted laser desorption/ionization time-of-flight collision-induced dissociation using poly(ethylene glycol)
Rapid communications in mass spectrometry, v 17(14), pp 1671-1676
2003
PMID: 12845595
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Details of the optimization of the collision-induced dissociation (CID) process, using a collision cell on a matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometer, are described using poly(ethylene glycol) 1000 (PEG 1000) as a model analyte. The effects of collision gas identity (helium, air, and argon), as well as collision gas pressure, on the resulting MS/MS data were investigated. With PEG 1000, helium was found to give the best results with respect to signal-to-noise (S/N) ratio. The optimum pressure for each gas was found to be in the range where the precursor ion signal was attenuated to approximately 30-50% for helium and 40-60% for argon. The effect of cation choice (Li, Na, and K) on the CID of PEG was also studied. CID spectra were produced for each, but PEG cationized with lithium was found to produce the spectra with the highest S/N ratio. The MALDI-TOF CID spectra that were generated for PEG were compared with the high-energy and low-energy MS/MS spectra obtained from a sector mass spectrometer and from a triple quadrupole mass spectrometer, respectively. The results observed for PEG confirm that CID on a MALDI-TOF mass spectrometer is a high-energy MS/MS technique.
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Details
- Title
- Optimization of matrix-assisted laser desorption/ionization time-of-flight collision-induced dissociation using poly(ethylene glycol)
- Creators
- Andrew J Hoteling - Drexel UniversityKenji Kawaoka - Imaging Materials and Media, R&D, Eastman Kodak Company, Rochester, NY 14650-2132, USA.Mary C Goodberlet - Imaging Materials and Media, R&D, Eastman Kodak Company, Rochester, NY 14650-2132, USA.Wan-Mo Yu - Imaging Materials and Media, R&D, Eastman Kodak Company, Rochester, NY 14650-2132, USA.Kevin G Owens - Drexel University
- Publication Details
- Rapid communications in mass spectrometry, v 17(14), pp 1671-1676
- Publisher
- Wiley
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000184039400021
- Scopus ID
- 2-s2.0-0037594449
- Other Identifier
- 991019168861804721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- Spectroscopy