Journal article
Optimization of secondary chemical equilibria in liquid chromatography: variables influencing the self-selectivity, retention, and efficiency in acid-base systems
Analytical chemistry (Washington), v 59(1), pp 110-115
01 Jan 1987
Abstract
The effects of several chromatographic variables on the self-selectivity (/r/AX/#r,A), retention, and efficiency In secondary chemical equilibria liquid chromatography (SCE-LC) are reported. The self-selectIvltles were measured for numerous ionogenlc compounds using pH-buffered mobile phases. Only the type of stationary phase employed affected the self-selectivity by more than a factor of 2. Data from the plots used to measure the seH-selectlvKIes are Included and serve as additional verification of the SCE-LC retention model discussed In the preceding paper. Solute retention can be varied without affecting the overall chromatographic selectivity by changing the amount of organic solvent added to the aqueous mobile phase or the chain length of the stationary phase. Column efficiency can be Improved by factors of 3-15 by adding small amounts of organic solvent to the aqueous mobile phase; this does not significantly affect the selectivity. When >4% 1-propanol Is used, the resulting efficiencies are equal to those obtained in conventional liquid chromatography. An approach for the optimization of resolution Is summarized
Metrics
Details
- Title
- Optimization of secondary chemical equilibria in liquid chromatography: variables influencing the self-selectivity, retention, and efficiency in acid-base systems
- Creators
- Joe P Foley - National Institute of Standards and TechnologyWillie E May - National Institute of Standards and Technology
- Publication Details
- Analytical chemistry (Washington), v 59(1), pp 110-115
- Publisher
- American Chemical Society
- Number of pages
- 6
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:A1987F379200024
- Scopus ID
- 2-s2.0-0023089795
- Other Identifier
- 991021906514504721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Analytical