Journal article
Optimization of secondary chemical equilibria in liquid chromatography: theory and verification
Analytical chemistry (Washington), v 59(1), pp 102-109
01 Jan 1987
Abstract
A general theory for the optimization of secondary chemical equilibria In liquid chromatography (SCE-LC) Is presented. Equations that predict the optimum mobile phase conditions are derived explicitly for the equilibrium AX ^ A + X. The selectivity of SCE-LC has been substantially underestimated because non-optimum mobile phase conditions were employed in previous selectivity estimates. An upper bound for the selectivity Is given by a < 10| |, where ApK Is the difference In the equilibrium constants of the two solutes. The self-selectivity, or ratio of capacity factors, */AX/*/A, deter-mines the mobile phase optimum and the overall chromato-graphic selectivity which can be achieved. The maximum selectivity Is predicted for several values of k' ^/k' A and K. The optimum concentration of equilibrant, [X]0PT, can be predicted from = * + (1/2) log (*'AX/#r'A),where pXotn = -log [X]ot and P^avg is the average effective equilibrium constant of the two solutes. The theory Is experimentally verified for acid-base equilibria.
Metrics
Details
- Title
- Optimization of secondary chemical equilibria in liquid chromatography: theory and verification
- Creators
- Joe P Foley - National Institute of Standards and TechnologyWillie E May - National Institute of Standards and Technology
- Publication Details
- Analytical chemistry (Washington), v 59(1), pp 102-109
- Publisher
- American Chemical Society
- Number of pages
- 8
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:A1987F379200023
- Scopus ID
- 2-s2.0-0023133011
- Other Identifier
- 991021906514604721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Analytical