Journal article
Optimizing Bifurcated Channels within an Anisotropic Scaffold for Engineering Vascularized Oriented Tissues
Advanced healthcare materials, v 9(24), pp 1-9
01 Dec 2020
PMID: 32790048
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Despite progress in engineering both vascularized tissues and oriented tissues, the fabrication of 3D vascularized oriented tissues remains a challenge due to an inability to successfully integrate vascular and anisotropic structures that can support mass transfer and guide cell alignment, respectively. More importantly, there is a lack of an effective approach to guiding the scaffold design bearing both structural features. Here, an approach is presented to optimize the bifurcated channels within an anisotropic scaffold based on oxygen transport simulation and biological experiments. The oxygen transport simulation is performed using the experimentally measured effective oxygen diffusion coefficient and hydraulic permeability of the anisotropic scaffolds, which are also seeded with muscle precursor cells and cultured in a custom-made perfusion bioreactor. Symmetric bifurcation model is used as fractal unit to design the channel network based on biomimetic principles. The bifurcation level of channel network is further optimized based on the oxygen transport simulation, which is then validated by DNA quantification assay and pimonidazole immunostaining. This study provides a practical guide to optimizing bifurcated channels in anisotropic scaffolds for oriented tissue engineering.
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Details
- Title
- Optimizing Bifurcated Channels within an Anisotropic Scaffold for Engineering Vascularized Oriented Tissues
- Creators
- Yongcong Fang - Tsinghua UniversityLiliang Ouyang - Imperial College LondonTing Zhang - Tsinghua UniversityChengjin Wang - Tsinghua UniversityBingchuan Lu - Tsinghua UniversityWei Sun - Tsinghua University
- Publication Details
- Advanced healthcare materials, v 9(24), pp 1-9
- Publisher
- Wiley
- Number of pages
- 9
- Grant note
- 2018YFA0703004 / National Key Research and Development Program of China 31771108 / National Natural Science Foundation of China (NSFC)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000558666600001
- Scopus ID
- 2-s2.0-85089396464
- Other Identifier
- 991019167644904721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials
- Nanoscience & Nanotechnology