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Optogenetic dissection reveals multiple rhythmogenic modules underlying locomotion
Journal article   Open access   Peer reviewed

Optogenetic dissection reveals multiple rhythmogenic modules underlying locomotion

Martin Hägglund, Kimberly J. Dougherty, Lotta Borgius, Shigeyoshi Itohara, Takuji Iwasato and Ole Kiehn
Proceedings of the National Academy of Sciences - PNAS, v 110(28), pp 11589-11594
24 Jun 2013
PMID: 23798384
url
https://www.pnas.org/content/pnas/110/28/11589.full.pdfView
Published, Version of Record (VoR) Open
url
https://doi.org/10.1073/pnas.1304365110View
Published, Version of Record (VoR) Open

Abstract

Biological Sciences channelrhodopsin-2 halorhodopsin interneurons motor neurons
Neural networks in the spinal cord known as central pattern generators produce the sequential activation of muscles needed for locomotion. The overall locomotor network architectures in limbed vertebrates have been much debated, and no consensus exists as to how they are structured. Here, we use optogenetics to dissect the excitatory and inhibitory neuronal populations and probe the organization of the mammalian central pattern generator. We find that locomotor-like rhythmic bursting can be induced unilaterally or independently in flexor or extensor networks. Furthermore, we show that individual flexor motor neuron pools can be recruited into bursting without any activity in other nearby flexor motor neuron pools. Our experiments differentiate among several proposed models for rhythm generation in the vertebrates and show that the basic structure underlying the locomotor network has a distributed organization with many intrinsically rhythmogenic modules.

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Web of Science research areas
Neurosciences
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