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Organization and expression of endogenous murine mammary tumor virus genes in mice congenic at the H-2 complex
Journal article   Peer reviewed

Organization and expression of endogenous murine mammary tumor virus genes in mice congenic at the H-2 complex

Carole A Long, Umakant J Dumaswala, Stephen L Tancin and Akhil B Vaidya
Virology (New York, N.Y.), v 103(1), pp 167-177
1980
DOI: https://doi.org/10.1016/0042-6822(80)90135-X
PMID: 6245525
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

The organization and expression of endogenous murine mammary tumor virus (MuMTV) genetic information was examined in a number of mouse strains Congenic at the major histocompatibility complex (MHC). Analysis of restriction endonuclease-generated fragments of cellular DNA revealed no differences in the integration sites ( EcoRI)or internal cleavage sites ( PstI) of MuMTV proviruses in any of the (C7BL/10 (B10) Congenic strains tested. Similar studies indicated that the proviruses of BALB/c and BALB.B mice are indistinguishable, and that the restriction endonuclease-generated fragments of BALB/c mice are similar to those of the B10 strain. These proviruses behave as stable genetic elements which have been well conserved during the derivation of the Congenic strains. Also, since none of the characteristic MuMTV proviruses of the donor strains could be identified in the Congenic mice, they do not seem to be closely linked to the H-2 complex. When concentrations of viral RNA in lactating mammary glands (LMGs) were measured by molecular hybridization kinetics, comparable levels of MuMTV RNA were found in all the B10 Congenic mice; considerably less viral RNA was detected in mice with a BALB/c background. This viral RNA in mammary gland cells was not translated into detectable gp49, the major MuMTV glycoprotein. These results indicate that the differences observed in susceptibility to mammary tumorigenesis induced in H-2 Congenic mice by exogenous MuMTV are not influenced by the organization or expression of endogenous MuMTV proviral genes.

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