Journal article
Organophosphorus hydrolase multilayer modified microcantilevers for organophosphorus detection
Biosensors & bioelectronics, v 22(11), pp 2636-2642
2007
PMID: 17140787
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We report a biosensor based on organophosphorus hydrolase (OPH) multilayer modified microcantilever (MCL) for detection of organophosphorus compounds (OPs). The assay is based on substrate-dependent bending of the OPH functionalized MCLs. The cantilever bending amplitude at equilibrium was a function of the concentration of paraoxon with the dynamic range extending from 10
−7 to 10
−3
M. The lower detection limit of approximately 10
−7
M for paraoxon was an order of magnitude better than the OPH-based potentiometric and optical biosensors based on pH modulation. There was a good intra-sensor and an acceptable inter-sensor reproducibility as evidenced by the standard errors of 5% and 15%, respectively. OPs measured using this technique included parathion and diisopropyl fluorophosphate (DFP) in the order of paraoxon
>
DFP
>
parathion. The conformational change of the OPH was most likely the main origin of MCL bending.
Metrics
Details
- Title
- Organophosphorus hydrolase multilayer modified microcantilevers for organophosphorus detection
- Creators
- Chandana Karnati - Department of Chemistry, Institute for Micromanufacturing, Louisiana Tech University, Ruston, LA 71272, United StatesHongwei Du - Department of Chemistry, Institute for Micromanufacturing, Louisiana Tech University, Ruston, LA 71272, United StatesHai-Feng Ji - Department of Chemistry, Institute for Micromanufacturing, Louisiana Tech University, Ruston, LA 71272, United StatesXiaohe Xu - Department of Chemistry, Institute for Micromanufacturing, Louisiana Tech University, Ruston, LA 71272, United StatesYuri Lvov - Department of Chemistry, Institute for Micromanufacturing, Louisiana Tech University, Ruston, LA 71272, United StatesAshok Mulchandani - Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, United StatesPriti Mulchandani - Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, United StatesWilfred Chen - Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, United States
- Publication Details
- Biosensors & bioelectronics, v 22(11), pp 2636-2642
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000246382500035
- Scopus ID
- 2-s2.0-33947592590
- Other Identifier
- 991014878649604721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biophysics
- Biotechnology & Applied Microbiology
- Chemistry, Analytical
- Electrochemistry
- Nanoscience & Nanotechnology