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Journal article
Overexpressed Full-Length Human BCL2 Extends the Survival of Baculovirus Infected Sf9 Insect Cells
Proceedings of the National Academy of Sciences - PNAS, v 89(16), pp 7295-7299
15 Aug 1992
PMID: 1502141
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Abstract
Full-length and truncated human BCL2 lacking the entire C-terminal hydrophobic domain have been overexpressed in Spodoptera frugiperda insect cells with the baculovirus expression system. Immunoblot analysis with BCL2-specific antibodies revealed that both full-length and truncated BCL2 are expressed as multiple immunoreactive species, suggesting posttranslational modifications. The expression of the full-length but not the truncated BCL2 extended the survival of baculovirus-infected cells by preventing virus-induced DNA cleavage. This result is consistent with the reported protective effect of BCL2 against apoptosis in mammalian lymphocytes and suggests a conserved function in evolution. Subcellular fractionation and indirect immunofluorescence studies in intact cells demonstrated that the recombinant full-length and truncated BCL2 proteins were expressed predominantly as nuclear membrane-associated proteins. These results imply that BCL2 must utilize hydrophobic domains other than the deleted domain for its association with the subcellular membranes. Metabolic labeling of insect cells expressing the full-length and the truncated form of BCL2 with32Pidemonstrated that BCL2 is a phosphoprotein.
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Details
- Title
- Overexpressed Full-Length Human BCL2 Extends the Survival of Baculovirus Infected Sf9 Insect Cells
- Creators
- Emad S. AlnmriNoreen M. Robertson - Thomas Jefferson UniversityTeresa F. Fernandes - Thomas Jefferson UniversityCarlo M. Croce - Thomas Jefferson UniversityGerald Litwack - Thomas Jefferson University
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, v 89(16), pp 7295-7299
- Publisher
- National Academy of Sciences of the United States of America
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- SOM Dean - Research Administration
- Web of Science ID
- WOS:A1992JJ32100003
- Scopus ID
- 2-s2.0-0026774213
- Other Identifier
- 991021463662204721
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- Web of Science research areas
- Biochemistry & Molecular Biology