Journal article
Overexpression of Fibulin-5 Attenuates Ischemia/Reperfusion Injury After Middle Cerebral Artery Occlusion in Rats
Molecular neurobiology, v 53(5), pp 3154-3167
01 Jul 2016
PMID: 26021746
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Ischemia/reperfusion (I/R) injury after middle cerebral artery occlusion (MCAO) induces detrimental processes such as oxidative stress, inflammation, and apoptosis. All parts of the neurovascular unit are involved in these pathological processes. Fibulin-5 is a 66-kD glycoprotein secreted by various vascular cells, including vascular smooth muscle cells (SMCs), fibroblasts, and endothelial cells. As an extracellular matrix protein involved in cell adhesion, fibulin-5 has been widely studied in tumor growth and invasion. However, the effects of fibulin-5 on brain injury following ischemia/reperfusion have not been reported. In this study, we examined the effect of overexpressed fibulin-5 on reactive oxygen species (ROS) production. Fibulin-5 overexpression attenuated ROS expression, which in turn decreased apoptosis and blood-brain barrier (BBB) permeability following MCAO and reperfusion. Fibulin-5 also improved neurological deficits but had no effect on infarction volume. T2-weighted MRI and electron microscopy further confirmed brain edema reduction and decreased BBB disruption in fibulin-5 overexpression recombinant adenovirus (Ad-FBLN) treated rats. In addition, tight junction protein occludin was significantly degraded and matrix metalloproteinase 9 (MMP-9) immunoreactivity was significantly increased. Fibulin-5-mediated ROS decrease was not due to increased total superoxide dismutase levels but was instead correlated with the activation of Rac-1 pathway. The findings highlight the importance of antioxidant mechanism underlying cerebral ischemia/reperfusion.
Metrics
Details
- Title
- Overexpression of Fibulin-5 Attenuates Ischemia/Reperfusion Injury After Middle Cerebral Artery Occlusion in Rats
- Creators
- Jia Guo - First Affiliated Hospital of Chongqing Medical UniversityChuang Cheng - First Affiliated Hospital of Chongqing Medical UniversityCindy Si Chen - Drexel UniversityXiangfeng Xing - First Affiliated Hospital of Chongqing Medical UniversityGuanghui Xu - First Affiliated Hospital of Chongqing Medical UniversityJinzhou Feng - First Affiliated Hospital of Chongqing Medical UniversityXinyue Qin - First Affiliated Hospital of Chongqing Medical UniversityJu-Tao Guo - Microbiology and Immunology
- Publication Details
- Molecular neurobiology, v 53(5), pp 3154-3167
- Publisher
- Humana Press Inc
- Number of pages
- 14
- Grant note
- 81271307 / General Program of National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000377935400037
- Scopus ID
- 2-s2.0-84930154352
- Other Identifier
- 991019173521404721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Neurosciences