Journal article
Overexpression of SR proteins and splice variants modulates chondrogenesis
Experimental cell research, v 313(8), pp 1509-1517
2007
PMID: 16140295
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Fibronectin alternative exon EIIIA is largely included in undifferentiated mesenchymal cells of the developing limb bud, whereas the exon is excluded in differentiated chondrocytes. Inclusion of exon EIIIA in chondrocytic cells is increased by overexpression of SRp40, and, to a lesser extent, SRp75, but not SRp55. RT-PCR analysis using real-time PCR revealed that the levels of the mRNAs for these three proteins did not vary significantly in chick chondrocytes versus mesenchymal cells of the developing limb bud. However, a variant spliced form of SRp40, termed, SRp40LF, is detected preferentially in chondrocytes and in chondrifying mesenchymal cells. Forced overexpression of SRp40 or SRp75, but not SRp55, enhanced chondrogenic differentiation of chick limb mesenchymal cells in a high-density micromass assay. Overexpression of SRp40LF, which produces a truncated form of SRp40, also was strongly pro-chondrogenic. In a HeLa cell-based assay, SRp40LF fails to substitute for SRp40 in mediating an increase in exon EIIIA inclusion, suggesting that the latter event is not essential for the pro-chondrogenic effect. These results demonstrate the ability of these highly conserved splicing factors to modulate chondrogenesis and are consistent with earlier results that implicated exon EIIIA-containing isoforms of fibronectin in formation of chondrogenic condensations.
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Details
- Title
- Overexpression of SR proteins and splice variants modulates chondrogenesis
- Creators
- Hongyan Liang - Department of Biochemistry and Molecular Pharmacology, and Jefferson Center for Biomedical Research, Thomas Jefferson University, Philadelphia, PA 19107, USARocky S Tuan - Cartilage Biology and Orthopaedics Branch, National Institute of Arthritis, and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USAPamela A Norton - Department of Biochemistry and Molecular Pharmacology, and Jefferson Center for Biomedical Research, Thomas Jefferson University, Philadelphia, PA 19107, USA
- Publication Details
- Experimental cell research, v 313(8), pp 1509-1517
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000246128300001
- Scopus ID
- 2-s2.0-34247119291
- Other Identifier
- 991014877769404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology
- Oncology