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Journal article
Overexpression of XIAP Inhibits Apoptotic Cell Death in an Oligodendroglial Cell Line
Cellular and molecular neurobiology, v 24(6), pp 853-863
Dec 2004
PMID: 15672685
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
1. This study describes the use of an oligodendroglial cell line (158N) to study the protective effects of X-chromosome-linked inhibitor of apoptosis (XIAP) overexpression.2. 158N cells were transiently transfected with either pCMV-Myc-XIAP or control pCMV-Myc vector. At 48 h post-transfection, immunoblotting and immunocytochemical staining showed robust myc-XIAP overexpression in pCMV-Myc-XIAP transfected cells relative to pCMV-Myc-transfected cells and normal 158N cells. 158N cells were treated with either 100 nm staurosporine (STS) or 300 μM dopamine (DA) and cell survival/function determined using two cell viability assays.3. Both STS and DA treatments resulted in increased apoptotic death of pCMV-Myc transfected cells. In contrast, there was significant decrease in apoptotic cell death in cells transfected with pCMV-Myc-XIAP. Finally, XIAP overexpression was found to significantly reduce caspase-3 enzyme activity levels in response to apoptotic stimuli.4. These results provide evidence that XIAP overexpression promotes cell survival in a non-neuronal cell type derived from the central nervous system. In addition, these data suggest that the 158N oligodendroglial cell line is a suitable tool for transient transfection studies, which is a problem frequently encountered when attempting to introduce genes of interest in cultures of primary oligodendroglia.
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Details
- Title
- Overexpression of XIAP Inhibits Apoptotic Cell Death in an Oligodendroglial Cell Line
- Creators
- Ying Jin - Departments of Anatomy and Neurobiology, Spinal Cord and Brain Injury Research Center University of Kentucky Medical Center Lexington KY 40536-0298Melanie McEwen - Departments of Anatomy and Neurobiology, Spinal Cord and Brain Injury Research Center University of Kentucky Medical Center Lexington KY 40536-0298M Ghandour - Institut de Physique Biologique, CNRS Strasbourg France 67085Joe Springer - Physical Medicine and Rehabilitation University of Kentucky College of Medicine, and Cardinal Hill Rehabilitation Hospital Lexington KY 40536-0298
- Publication Details
- Cellular and molecular neurobiology, v 24(6), pp 853-863
- Publisher
- Kluwer Academic Publishers-Plenum Publishers; New York
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000226091700012
- Scopus ID
- 2-s2.0-12944303668
- Other Identifier
- 991014877874304721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell Biology
- Neurosciences