Journal article
Overexpression of alpha 1,6-fucosyltransferase in hepatoma enhances expression of Golgi phosphoprotein 2 in a fucosylation-independent manner
International journal of oncology, v 39(1), pp 203-208
01 Jul 2011
PMID: 21503570
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Golgi phosphoprotein 2 (GP73) is a type IT Golgi protein, which was found on examination of the fucosylated proteome as a potential tumor marker for hepatocellular carcinoma (HCC). The serum levels of both total and fucosylated GP73 were increased in the sera of patients with HCC. Fucosylation is one of the most important oligosaccharide modifications involved in cancer and is catalyzed by alpha 1,6-fucosyltransferase (Fut8). In the present study, we investigated the effect of Fut8 overexpression on GP73 production in the human hepatoma cell line Hep3B. The Fut8 expression vector was transfected into Hep3B cells and the expression of GP73 was investigated by Western blotting and real-time PCR. Overexpression of Fut8 dramatically enhanced the expression of GP73 at the transcriptional level. Surprisingly, this effect was not dependent on cellular fucosylation. Overexpression of a mutant Fut8, which was unable to be localized to the Golgi, did not induce GP73 production, suggesting that the localization of Fut8 in the Golgi apparatus was important for the increase in GP73 expression. This is the first demonstration of GP73 regulation through overexpression of a glycosyltransferase, which may lead to Golgi stress.
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Details
- Title
- Overexpression of alpha 1,6-fucosyltransferase in hepatoma enhances expression of Golgi phosphoprotein 2 in a fucosylation-independent manner
- Creators
- Sayuri Kawamoto - Osaka UniversityKenta Moriwaki - Osaka Univ, Dept Mol Biochem & Clin Invest, Grad Sch Med, Suita, Osaka 5650871, JapanTsutomu Nakagawa - Osaka Univ, Dept Mol Biochem & Clin Invest, Grad Sch Med, Suita, Osaka 5650871, JapanMika Terao - Osaka Univ, Dept Mol Biochem & Clin Invest, Grad Sch Med, Suita, Osaka 5650871, JapanShinichiro Shinzaki - Osaka Univ, Dept Mol Biochem & Clin Invest, Grad Sch Med, Suita, Osaka 5650871, JapanNaoko Yamane-Ohnuki - Kyowa Hakko Kirin Co Ltd, Tokyo Res Labs, Tokyo, JapanMitsuo Satoh - Kyowa Hakko Kirin Co Ltd, Tokyo Res Labs, Tokyo, JapanAnand S. Mehta - Drexel Univ, Coll Med, Doylestown, PA USATimothy M. Block - Drexel UniversityEiji Miyoshi - Osaka University
- Publication Details
- International journal of oncology, v 39(1), pp 203-208
- Publisher
- Spandidos Publ Ltd
- Number of pages
- 6
- Grant note
- Ministry of Education, Culture, Sports, Science, and Technology of Japan; Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT) New Energy and Industrial Technology Development Organization (NEDO) 22790643 / Grants-in-Aid for Scientific Research; Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT); Japan Society for the Promotion of Science; Grants-in-Aid for Scientific Research (KAKENHI) R01 CA120206; R01 CA136607 / National Cancer Institute (NCI); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) 21249038 / Japan Society for the Promotion of Science; Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT) R01CA136607 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000291504900023
- Scopus ID
- 2-s2.0-79956052012
- Other Identifier
- 991019167637104721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Oncology