Journal article
Overexpression of succinyl-CoA synthase for poly (3-hydroxybutyrate-co-3-hydroxyvalerate) production in engineered Escherichia coli BL21(DE3)
Journal of applied microbiology, v 119(3), pp 724-735
01 Sep 2015
PMID: 26109231
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
AimThis study aims to increase the 3-hydroxyvalerate (3HV) fraction in poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(HB-co-HV)] using succinyl-CoA synthase.
Methods and ResultsEscherichia coliYH090, a polyhydroxyalkonate (PHA)-producing strain, was further engineered for overexpression of succinyl-CoA synthase genes (sucCD), and examined for P(HB-co-HV) copolymer production in the presence of various precursor molecules using mixture analysis. Glycerol, succinate and propionate were screened as important factors for controlling intracellular PHA accumulation and monomer composition. Glycerol concentrations exerted the greatest influence on the overall biomass concentration and the intracellular PHA content, while propionate concentrations in the presence of succinate influenced the 3HV content of the copolymer. Mixture analysis also demonstrated that the engineered strain has the capacity to accumulate up to 80% of its cell dry weight (CDW) as PHA with a variable fraction of 3HV monomer (maximum of 72 wt %) depending on the controlled conditions.
ConclusionsPropionate is the principal precursor for 3HV monomer in P(HB-co-HV) biopolymer and its utilization requires conversion to propionyl-CoA. Engineered E.coliYHY99, overexpressing sucCD genes, leads to an increase of the succinyl-CoA pool, which enhances the conversion rate of propionate by providing a CoA supply to other acyltransferase enzymes that have a role in propionate utilization.
Significance and Impact of the StudyEngineered E.coliYHY99 was able to utilize propionate with a 45-fold increase in rate, as compared to the control strain, and resulted in the synthesis of a copolymer with high 3HV monomer content.
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Details
- Title
- Overexpression of succinyl-CoA synthase for poly (3-hydroxybutyrate-co-3-hydroxyvalerate) production in engineered Escherichia coli BL21(DE3)
- Creators
- S. K. Bhatia - Konkuk UniversityD. -H. Yi - Konkuk UniversityH. -J. Kim - Konkuk UniversityJ. -M. Jeon - Konkuk UniversityY. -H. Kim - Konkuk UniversityG. Sathiyanarayanan - Konkuk UniversityH. M. Seo - Konkuk UniversityJ. H. Lee - Konkuk UniversityJ. -H. Kim - Konkuk UniversityK. Park - Hongik UniversityC. J. Brigham - University of Massachusetts DartmouthY. -H. Yang - Konkuk University
- Publication Details
- Journal of applied microbiology, v 119(3), pp 724-735
- Publisher
- Wiley
- Number of pages
- 12
- Grant note
- 20133030000300 / Energy Efficiency & Resources of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant - Korea Government Ministry of Trade, Industry and Energy NRF-2013R1A1A2A10004690; NRF-2011-619-E0002; NRF-2015R1A2A2A04006014 / Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education KU Brain Pool of Konkuk University Bio & Medical Technology Development Program of the NRF - Korean government 2015M3A9B8031831 / MSIP
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000360231000011
- Scopus ID
- 2-s2.0-84939565791
- Other Identifier
- 991021229991504721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Microbiology