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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Oxygen Microscopy by Two-Photon-Excited Phosphorescence
Chemphyschem, v 9(12), pp 1673-1679
25 Aug 2008
PMID: 18663708
Abstract
High-resolution images of oxygen distributions in microheterogeneous samples are obtained by two-photon laser scanning microscopy (2P LSM), using a newly developed dendritic nanoprobe with internally enhanced two-photon absorption (2PA) cross-section. In this probe, energy is harvested by a 2PA antenna, which passes excitation onto a phosphorescent metalloporphyrin via intramolecular energy transfer. The 2P LSM allows sectioning of oxygen gradients with near diffraction-limited resolution, and lifetime-based acquisition eliminates dependence on the local probe concentration. The technique is validated on objects with a priori known oxygen distributions and applied to imaging of pO
2
in cells.
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Details
- Title
- Oxygen Microscopy by Two-Photon-Excited Phosphorescence
- Creators
- Olga S. Finikova - Department of Biochemistry and Biophysics, University of Pennsylvannia, Philadelphia PA 19104‐6059 (USA), Fax: (+1) 215‐573‐3787Artem Y. Lebedev - Department of Biochemistry and Biophysics, University of Pennsylvannia, Philadelphia PA 19104‐6059 (USA), Fax: (+1) 215‐573‐3787Alexey Aprelev - Drexel UniversityThomas Troxler - Department of Biochemistry and Biophysics, University of Pennsylvannia, Philadelphia PA 19104-6059 (USA), Fax: (+1) 215-573-3787 Department of Physics, Drexel University, Philadelphia, PA 19104 (USA) Department of Chemistry and RLBL, University of Pennsylvannia, Philadelphia, PA 19104 (USA) Department of Pharmacology, University of Pennsylvania, Philadelphia, PA 19104 (USA)Feng Gao - Department of Chemistry and RLBL, University of Pennsylvannia, Philadelphia, PA 19104 (USA)Carmen Garnacho - University of PennsylvaniaSilvia Muro - University of PennsylvaniaRobin M. Hochstrasser - Department of Chemistry and RLBL, University of Pennsylvannia, Philadelphia, PA 19104 (USA)Sergei A. Vinogradov - Department of Biochemistry and Biophysics, University of Pennsylvannia, Philadelphia PA 19104‐6059 (USA), Fax: (+1) 215‐573‐3787
- Publication Details
- Chemphyschem, v 9(12), pp 1673-1679
- Publisher
- Wiley
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Physics
- Web of Science ID
- WOS:000258851200003
- Scopus ID
- 2-s2.0-51249083709
- Other Identifier
- 991019169554404721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Physical
- Physics, Atomic, Molecular & Chemical