Journal article
Oxygen Sensing and HIF-1 Activation Does Not Require an Active Mitochondrial Respiratory Chain Electron-transfer Pathway
The Journal of biological chemistry, v 276(25), pp 21995-21998
22 Jun 2001
PMID: 11342528
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Hypoxia induces the stabilization and transcriptional activation of the hypoxia-inducible factor 1
α
(HIF-1
α
) protein, the regulatory member of the HIF-1 complex. The molecular mechanisms that are responsible for oxygen sensing and the downstream pathways utilized by the hypoxic signal are still poorly understood. One hypothesis for oxygen sensing has postulated that reactive oxygen species generated at mitochondrial complex III are the initiators of the hypoxic signal. Here we find that mitochondrial DNA-less (
ρ
o
) cells have a normal response to hypoxia, measured at the level of HIF-1
α
protein stabilization, nuclear translocation, and its transcriptional activation activity. Furthermore, overexpression of catalase, either in the mitochondria or in the cytosol, fails to modify the hypoxia response indicating that hydrogen peroxide is not a signaling molecule in the hypoxic signaling cascade that culminates with HIF-1 activation.
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Details
- Title
- Oxygen Sensing and HIF-1 Activation Does Not Require an Active Mitochondrial Respiratory Chain Electron-transfer Pathway
- Creators
- Vickram SrinivasIrene LeshchinskyNianli SangMichael P King - Department of Biochemistry and Molecular Pharmacology, Jefferson Medical College of Thomas Jefferson University, Philadelphia, Pennsylvania 19107-5099Alex MinchenkoJaime Caro
- Publication Details
- The Journal of biological chemistry, v 276(25), pp 21995-21998
- Publisher
- ASBMB Publications / Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000169412700002
- Scopus ID
- 2-s2.0-0035933716
- Other Identifier
- 991014878467604721
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- Web of Science research areas
- Biochemistry & Molecular Biology