Journal article
P-11 Expression of a novel anti-apoptotic protein, survivin in glial cells of progressive multifocal leukoencephalopathy
Journal of neurovirology, Vol.11
01 Jan 2005
Abstract
Progressive Multifocal Leukoencephalopathy (PML) is a sub-acute and fatal disease of the Central Nervous System, result of the productive infection of glial cells, particularly oligodendrocytes, by the opportunistic JC Virus. JC Virus is a human neurotropic virus, prevalent in the adult population world wide, with approximately 85 to 90% of the population exhibiting hemmaglutinating antibodies. Primary infection is sub-clinical and the virus remains in latent state presumably in the kidney, until its reactivation under immunosuppressive conditions, particularly in AIDS patients, to result in the lytic destruction of oligodendrocytes and in the development of PML. The lytic destruction of oligodendrocytes and the activation of astrocytes in response to the injury caused by JCV infection results in the characteristic histopathological landmarks of PML; extensive and confluent areas of myelin loss, in which numerous bizarre reactive astrocytes with atypical and pleomorphic nuclei, and enlarged oligodendrocytes harboring intra-nuclear eosinophilic inclusion bodies. The natural response of the organism to dispose of damaged or infected cells is programmed cell death or apoptosis. The intricate mechanisms that will decide between cell death and cell survival, depend on a delicate balance between pro-apoptotic proteins and inhibitors of apoptosis. Survivin, a recently identified protein, is a member of the inhibitor of apoptosis gene family, which has been associated with apoptosis inhibition as well as cell mitosis regulation. This protein is abundantly expressed during development in embryonic proliferating tissues, but is absent in terminally differentiated cells. In addition, Survivin is selectively expressed in most of common human cancers, including tumors of glial and neural origin, in which it has been associated with poor prognosis. Immunohistochemical experiments performed in a collection of 18 brain samples from patients with AIDS related PML and 2 cases of non-AIDS PML, revealed increased expression of Survivin in bizarre reactive astrocytes and in the intra-nuclear inclusion bodies of JCV infected oligodendrocytes. In addition, JCV infected primary astrocytic cell cultures demonstrated enhanced expression of Survivin by Westen Blot and immunocytochemistry compared to non-infected cultures, which lack expression of Survivin.
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Details
- Title
- P-11 Expression of a novel anti-apoptotic protein, survivin in glial cells of progressive multifocal leukoencephalopathy
- Creators
- L Del ValleT SweetS Pina-OviedoS RadhakrishnanK Khalili
- Publication Details
- Journal of neurovirology, Vol.11
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Epidemiology and Biostatistics
- Identifiers
- 991021448047304721