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P248Sensitivity of the amsel's criteria compared to the nugent score in absence and in presence of trichomonas vaginalis (TV) and/or candida SPP among women with symptomatic vaginitis/vaginosis
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P248Sensitivity of the amsel's criteria compared to the nugent score in absence and in presence of trichomonas vaginalis (TV) and/or candida SPP among women with symptomatic vaginitis/vaginosis

Lucile Belley-Montfort, Joel Lebed, Bonnie Smith, Melissa Farrell, Jane Schwebke, Paul Nyirjesy, Thomas Davis, DeAnna Fuller, Kenneth Fife and Safedin Beqaj
Sexually transmitted infections, v 91(Suppl 1), pp A97-A97
01 Jun 2015
url
https://sti.bmj.com/content/91/Suppl_1/A97.2.full.pdfView
Published, Version of Record (VoR) Open
url
https://doi.org/10.1136/sextrans-2015-052126.290View
Published, Version of Record (VoR) Open

Abstract

Candida Trichomonas vaginalis
Background/introductionIn a multicenter clinical trial funded by BD, we observed less accurate clinician diagnosis of bacterial vaginosis (BV) based on clinical observations when Trichomonas vaginalis (TV) and/or Candida spp. were also detected by the trial Reference Methods than when only BV was detected.Aim(s)/objectivesTo determine the sensitivity of each criterion and of the overall Amsel's criteria (3/4 criteria met), the results of the Amsel's corresponding to the sub-population of specimens that gave a Nugent score of 7-10 were analysed.MethodsFollowing informed consent, women with symptoms of vaginitis/vaginosis were included in the trial. The four Amsel's criteria and the Nugent score were performed. Evaluation for trichomoniasis by wet mount and culture (InPouch(TM) TV, Biomed) were performed. Candida colonies were isolated (BBL(TM) Sabouraud Dextrose Agar, Emmons and BBL(TM) CHROMAgar(TM) Candida plate, BD) and identified by ITS-2 bi-directional sequencing (Accugenix registered ).ResultsIn total, 269/497 (54.1%) specimens gave a Nugent score of 7-10. Amongst them, TV and/or Candida spp. were found in 100 specimens (37.2%). The sensitivity of clue cells, amine test, vaginal pH, BV vaginal discharge, and overall Amsel's criteria in absence of TV and/or Candida spp. was 86.3%, 82.7%, 91.1%, 71.0%, and 84.6% respectively. In presence of TV and/or Candida spp., the sensitivity was 63.6%, 64.0%, 75.0%, 42.0%, and 60.0% respectively (p values less than or equal to 0.0009 for all comparisons).Discussion/conclusionThe sensitivity of the Amsel's criteria in women with BV decreases when TV and/or Candida spp. are present. The BV vaginal discharge is the least sensitive criterion.

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Collaboration types
Domestic collaboration
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Web of Science research areas
Infectious Diseases
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