Journal article
PEMC sensor's mass change sensitivity is 20 pg/Hz under liquid immersion
Biosensors & bioelectronics, v 22(1), pp 35-41
15 Jul 2006
PMID: 16387487
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
To enhance the mass change sensitivity of the resonating piezoelectric-excited millimeter-sized cantilever (PEMC) sensors, we reduced its length and eliminated one layer of its composite structure. As a result the mass sensitivity of the second flexural mode increased by two orders of magnitude (from 10(-9) to 10(-11)g/Hz) and the resonant frequency increased by more than 5 kHz. We demonstrate the effects of modification by detecting a model pathogen Group A Streptococcus (GAS) at 700 cells/mL. The resonant frequency change of the second mode at concentrations of 700, 7 x 10(3), 7 x 10(5), 7 x 10(6), 7 x 10(7), and 7 x 10(9)cells/mL resulted in, respectively, 3.1+/-0.5, 11.6+/-1, 15.7+/-1, 25.7+/-0.15, 28.5+/-2, and 40.5+/-3 ng (n=3 for all) of pathogen attachment. A kinetic model for the binding is proposed and verified. The observed binding rate constant was found to be in the range of 0.051-0.166 min(-1). The significance of the results we report is that the modified PEMC sensors have high mass sensitivity that pathogens can be detected at very low concentration under liquid immersion conditions.
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Details
- Title
- PEMC sensor's mass change sensitivity is 20 pg/Hz under liquid immersion
- Creators
- Gossett A Campbell - Department of Chemical and Biological Engineering, Drexel University, Philadelphia, PA 19104, USARaj Mutharasan
- Publication Details
- Biosensors & bioelectronics, v 22(1), pp 35-41
- Publisher
- Elsevier; England
- Grant note
- 5R01EB000720 / NIBIB NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000241040600005
- Scopus ID
- 2-s2.0-33746531720
- Other Identifier
- 991014877757504721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biophysics
- Biotechnology & Applied Microbiology
- Chemistry, Analytical
- Electrochemistry
- Nanoscience & Nanotechnology