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Journal article
PHENOTYPIC DIVERSITY AND EXPRESSION OF GABAergic INHIBITORY INTERNEURONS DURING POSTNATAL DEVELOPMENT IN LUMBAR SPINAL CORD OF GLUTAMIC ACID DECARBOXYLASE 67-GREEN FLUORESCENT PROTEIN MICE
Neuroscience, v 163(3), pp 909-919
20 Oct 2009
PMID: 19560523
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The synthesis enzyme glutamic acid decarboxylase (GAD65 or GAD67) identifies neurons as GABAergic. Recent studies have characterized the physiological properties of spinal cord GABAergic interneurons using lines of GAD67-green fluorescent protein (GFP) transgenic mice. A more complete characterization of their phenotype is required to better understand the role of this population of inhibitory neurons in spinal cord function. Here, we characterize the distribution of lumbar spinal cord GAD67-GFP neurons at postnatal days (P) 0, 7, and 14, and adult based on their co-expression with GABA and determine the molecular phenotype of GAD67-GFP neurons at P14 based on the expression of various neuropeptides, calcium binding proteins, and other markers. At all ages >67% of GFP(+) neurons were also GABA(+). With increasing age; (1) GFP(+) and GABA(+) cell numbers declined, (ii) ventral horn GFP(+) and GABA(+) neurons vanished, and (iii) somatic labeling was reduced while terminal labeling increased. At P14, vasoactive intestinal peptide and bombesin were expressed in similar to 63% and similar to 35% of GFP(+) cells, respectively. Somatostatin was found in a small number of neurons, whereas calcitonin gene-related peptide never co-localized with GFP. Moderate co-expression was found for all the Ca2+ binding proteins examined. Notably, most laminae I-II parvalbumin neurons were also GFP. Neurogranin, a protein kinase C substrate, was found in similar to 1/2 of GFP(+) cells. Lastly, while only 7% of GFP(+) cells contain nitric oxide synthase (NOS), these cells represent a large fraction of all NOS+ cells. We conclude that GAD67-GFP neurons represent the majority of spinal GABAergic neurons and that mouse dorsal horn GAD67-GFP(+) neurons comprise a phenotypically diverse population. (c) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.
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- Title
- PHENOTYPIC DIVERSITY AND EXPRESSION OF GABAergic INHIBITORY INTERNEURONS DURING POSTNATAL DEVELOPMENT IN LUMBAR SPINAL CORD OF GLUTAMIC ACID DECARBOXYLASE 67-GREEN FLUORESCENT PROTEIN MICE
- Creators
- K. J. Dougherty - Emory UniversityM. A. Sawchuk - Emory UniversityS. Hochman - Emory University
- Publication Details
- Neuroscience, v 163(3), pp 909-919
- Publisher
- Elsevier
- Number of pages
- 11
- Grant note
- R01NS045248 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) Christopher Reeve Foundation NS045248 / NINDS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) NS049784 / NINDS National Research Service Award; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy; College of Medicine; Drexel University
- Web of Science ID
- WOS:000270284900019
- Scopus ID
- 2-s2.0-70249087966
- Other Identifier
- 991020100061504721
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- Web of Science research areas
- Neurosciences