Journal article
PITALRE, a nuclear CDC2-related protein kinase that phosphorylates the retinoblastoma protein in vitro
Proceedings of the National Academy of Sciences - PNAS, v 91(9), pp 3834-3838
26 Apr 1994
PMID: 8170997
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Members of the cell division cycle 2 (CDC2) family of kinases play a pivotal role in the regulation of the eukaryotic cell cycle. In this communication, we report the isolation of a cDNA that encodes a CDC2-related human protein kinase temporarily designated PITALRE for the characteristic Pro-Ile-Thr-Ala-Leu-Arg-Glu motif. Its deduced amino acid sequence is 47% identical to that of the human cholinesterase-related cell division controller (CHED) kinase, which is required during hematopoiesis, and 42% identical to the Saccharomyces cerevisiae SGV1 gene product, a putative kinase involved in the response to pheromone via its guanine nucleotide-binding protein alpha subunit. PITALRE expression is ubiquitous, but its expression levels are different in various human tissues. PITALRE is an approximately 43-kDa protein that associates with three cellular polypeptides of 80, 95, and 155 kDa. PITALRE is localized primarily to the nucleus. In addition, we have identified a retinoblastoma protein kinase activity associated with PITALRE immunocomplexes that cannot phosphorylate histone H1, suggesting that the target phosphorylation site of PITALRE differs from that of CDC2 kinase. Interestingly, the retinoblastoma kinase activity associated with PITALRE does not oscillate during the cell cycle.
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Details
- Title
- PITALRE, a nuclear CDC2-related protein kinase that phosphorylates the retinoblastoma protein in vitro
- Creators
- X Graña - Fels Institute for Cancer Research and Molecular Biology, Department of Pathology, Temple University, School of Medicine, Philadelphia, PA 19140A De Luca - Fels Institute for Cancer Research and Molecular Biology, Department of Pathology, Temple University, School of Medicine, Philadelphia, PA 19140N Sang - Fels Institute for Cancer Research and Molecular Biology, Department of Pathology, Temple University, School of Medicine, Philadelphia, PA 19140Y Fu - Fels Institute for Cancer Research and Molecular Biology, Department of Pathology, Temple University, School of Medicine, Philadelphia, PA 19140P P Claudio - Fels Institute for Cancer Research and Molecular Biology, Department of Pathology, Temple University, School of Medicine, Philadelphia, PA 19140J Rosenblatt - Fels Institute for Cancer Research and Molecular Biology, Department of Pathology, Temple University, School of Medicine, Philadelphia, PA 19140D O Morgan - Fels Institute for Cancer Research and Molecular Biology, Department of Pathology, Temple University, School of Medicine, Philadelphia, PA 19140A Giordano - Fels Institute for Cancer Research and Molecular Biology, Department of Pathology, Temple University, School of Medicine, Philadelphia, PA 19140
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, v 91(9), pp 3834-3838
- Publisher
- PNAS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:A1994NJ03400075
- Scopus ID
- 2-s2.0-0028216325
- Other Identifier
- 991014877807504721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology