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POGO-DB—a database of pairwise-comparisons of genomes and conserved orthologous genes
Journal article   Open access   Peer reviewed

POGO-DB—a database of pairwise-comparisons of genomes and conserved orthologous genes

Yemin Lan, J. Calvin Morrison, Ruth Hershberg and Gail L Rosen
Nucleic acids research, v 42(Database issue), pp D625-D632
01 Jan 2014
PMID: 24198250
url
https://doi.org/10.1093/nar/gkt1094View
Published, Version of Record (VoR) Open

Abstract

IV. Viruses, bacteria, protozoa and fungi
POGO-DB ( http://pogo.ece.drexel.edu/ ) provides an easy platform for comparative microbial genomics. POGO-DB allows users to compare genomes using pre-computed metrics that were derived from extensive computationally intensive BLAST comparisons of >2000 microbes. These metrics include (i) average protein sequence identity across all orthologs shared by two genomes, (ii) genomic fluidity (a measure of gene content dissimilarity), (iii) number of ‘orthologs’ shared between two genomes, (iv) pairwise identity of the 16S ribosomal RNA genes and (v) pairwise identity of an additional 73 marker genes present in >90% prokaryotes. Users can visualize these metrics against each other in a 2D plot for exploratory analysis of genome similarity and of how different aspects of genome similarity relate to each other. The results of these comparisons are fully downloadable. In addition, users can download raw BLAST results for all or user-selected comparisons. Therefore, we provide users with full flexibility to carry out their own downstream analyses, by creating easy access to data that would normally require heavy computational resources to generate. POGO-DB should prove highly useful for researchers interested in comparative microbiology and benefit the microbiome/metagenomic communities by providing the information needed to select suitable phylogenetic marker genes within particular lineages.

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Domestic collaboration
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Web of Science research areas
Biochemistry & Molecular Biology
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