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POOL server: machine learning application for functional site prediction in proteins
Journal article   Open access   Peer reviewed

POOL server: machine learning application for functional site prediction in proteins

Srinivas Somarowthu and Mary Jo Ondrechen
Bioinformatics (Oxford, England), v 28(15), pp 2078-2079
01 Aug 2012
PMID: 22661648
url
https://doi.org/10.1093/bioinformatics/bts321View
Published, Version of Record (VoR) Open

Abstract

Biochemical Research Methods Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Computer Science Computer Science, Interdisciplinary Applications Life Sciences & Biomedicine Mathematical & Computational Biology Mathematics Physical Sciences Science & Technology Statistics & Probability Technology
We present an automated web server for partial order optimum likelihood (POOL), a machine learning application that combines computed electrostatic and geometric information for high-performance prediction of catalytic residues from 3D structures. Input features consist of THEMATICS electrostatics data and pocket information from ConCavity. THEMATICS measures deviation from typical, sigmoidal titration behavior to identify functionally important residues and ConCavity identifies binding pockets by analyzing the surface geometry of protein structures. Both THEMATICS and ConCavity (structure only) do not require the query protein to have any sequence or structure similarity to other proteins. Hence, POOL is applicable to proteins with novel folds and engineered proteins. As an additional option for cases where sequence homologues are available, users can include evolutionary information from INTREPID for enhanced accuracy in site prediction.

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30 citations in Scopus

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Web of Science research areas
Biochemical Research Methods
Biotechnology & Applied Microbiology
Computer Science, Interdisciplinary Applications
Mathematical & Computational Biology
Statistics & Probability
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