Journal article
PP2A(Cdc55) is required for multiple events during meiosis I
Cell cycle (Georgetown, Tex.), v 10(9), pp 1420-1434
01 May 2011
PMID: 21455032
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Protein phosphatase 2A (PP2A) is a heterotrimer consisting of A and B regulatory subunits and a C catalytic subunit. PP2A regulates mitotic cell events that include the cell cycle, nutrient sensing, p53 stability and various mitogenic signals. The role of PP2A during meiosis is less understood. We explored the role of Saccharomyces cerevisiae PP 2A during meiosis. We show a PP2A(Cdc55) containing the human B/55 family B subunit ortholog, Cdc55, is required for progression through meiosis I. Mutant cells lacking Cdc55 remain mononucleated. They harbor meiotic gene expression, premeiotic DNA replication, homologous recombination and spindle pole body (SPB) defects. They initiate but do not complete replication and are defective in performing intergenic homologous recombination. Bypass alleles, which allow cells defective in recombination to finish meiosis, do not suppress the meiosis I defect. cdc55 cells arrest with a single SPB lacking microtubules or duplicated but not separated SBPs containing microtubules. Finally, the premeiotic replication defect is suppressed by loss of Rad9 checkpoint function. We conclude PP2A(Cdc55) is required for the proper temporal initiation of multiple meiotic events and/or monitors these events to ensure their fidelity.
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Details
- Title
- PP2A(Cdc55) is required for multiple events during meiosis I
- Creators
- Jocelyn K. Nolt - Venenum Biodesign, Genesis Biotechnology Group, Hamilton, NJ, USA.Lyndi M. Rice - Venenum Biodesign, Hamilton, NJ USAChristina Gallo-Ebert - Venenum Biodesign, Hamilton, NJ USAMargaret E. Bisher - Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USAJoseph T. Nickels - Venenum Biodesign, Hamilton, NJ USA
- Publication Details
- Cell cycle (Georgetown, Tex.), v 10(9), pp 1420-1434
- Publisher
- Taylor & Francis
- Number of pages
- 15
- Grant note
- Drexel University College of Medicine Medical Diagnostic Laboratories
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000290145600021
- Scopus ID
- 2-s2.0-79955583900
- Other Identifier
- 991019173957804721
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- Web of Science research areas
- Cell Biology