Journal article
Patch Clamp Study of Serotonin-Gated Currents via 5-HT Type 3 Receptors by Using a Novel Approach SHAM for Receptor Channel Scanning
Journal of biomedicine & biotechnology, v 2004(1)
27 Apr 2004
PMID: 15123883
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We studied 5-hydroxy tryptamine type 3 (5-HT
3
) receptors
transfected in tsA-201 cell line to examine serotonin-induced
whole cell currents. Using the site-directed mutagenesis
technique, we individually mutated each residue in the
membrane-spanning M2 segment to histidine. A high proportion of
tsA-201 cells cotransfected with the cDNAs of 5-HT
3
R and CD8
produced large amplitude responses (0.5−7.0 nA) to
serotonin. The dose-response curve of wild-type (WT) receptor
ranging from 0.5 to 500
μ
mole increases its K
d
values, and V
max
of 5-HT
3
R falls at low external pH as if
protonation of an acid group is enough to block the channel.
Lysine at position 281, a basic residue, is more susceptible to
acidification-induced blockade of the 5-HT
3
R channel.
Dose-response curves of K281S (replacing lysine at the 281
position with serine) at different pH are not significantly
modulated, and histidine substitutions at the three consecutive
positions 293, 294, and 296 eliminate the pH block of the channel.
Metrics
Details
- Title
- Patch Clamp Study of Serotonin-Gated Currents via 5-HT Type 3 Receptors by Using a Novel Approach SHAM for Receptor Channel Scanning
- Creators
- Fatima-Shad Kaneez - Biology Department, Faculty of Science, University of UAE, PO Box 17551, Al Ain, United Arab EmiratesM White - Department of Pharmacology and Physiology, College of Medicine, Drexel University, Philadelphia, PA 19104, USA
- Publication Details
- Journal of biomedicine & biotechnology, v 2004(1)
- Publisher
- Hindawi Publishing Corporation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000221602100004
- Scopus ID
- 2-s2.0-3042764817
- Other Identifier
- 991014878091004721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Medicine, Research & Experimental