Journal article
Patterns of histone acetylation suggest dual pathways for gene activation by a bifunctional locus control region
The EMBO journal, v 19(24), pp 6814-6822
15 Dec 2000
PMID: 11118216
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The five genes of the human growth hormone (hGH) cluster are expressed in either the pituitary or placenta. Activation of the cluster is dependent on a locus control region (LCR) comprising pituitary- specific (HSI,II, –15 kb), placenta-specific (HSIV, –30 kb) and shared (HSIII, –28 kb; HSV, –32 kb) DNase I hypersensitive sites. Gene activation in the pituitary is paralleled by acetylation of a 32 kb chromatin domain 5′ to the cluster centered at HSI,II. In the present study we observed that acetylation of this region in placental chromatin was discretely limited to shared HSIII and HSV. Transgenic studies revealed placenta-specific activation of linked genes by a determinant (P-element) located 2 kb 5′ to each of the four placentally expressed genes. A localized peak of histone acetylation was observed at these P-elements in placenta but not pituitary. These data support a model for bifunctional action of the hGH LCR in which separate positive determinants, HSI,II and the P-elements, activate their respective target genes by tissue-specific recruitment of distinctly regulated histone acetyl transferase activities.
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Details
- Title
- Patterns of histone acetylation suggest dual pathways for gene activation by a bifunctional locus control region
- Creators
- Felice Elefant - Departments ofYuhua Su - Departments ofStephen A Liebhaber - Departments ofNancy E Cooke - Departments of
- Publication Details
- The EMBO journal, v 19(24), pp 6814-6822
- Publisher
- Oxford University Press; Oxford, UK
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000166052800019
- Scopus ID
- 2-s2.0-0034671798
- Other Identifier
- 991014877682504721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology