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Journal article
Performance of Four Fosfomycin Susceptibility Testing Methods against an International Collection of Clinical Pseudomonas aeruginosa Isolates
Journal of clinical microbiology, v 58(10)
01 Oct 2020
PMID: 32669380
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Fosfomycin has been shown to have a wide spectrum of activity against multidrug-resistant Gram-negative bacteria; however, breakpoints have been established only for Escherichia coli or Enterobacterates per the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibility Testing (EUCAST), respectively. A lack of additional organism breakpoints limits clinical use of this agent and has prompted extrapolation of these interpretive categories to other organisms like Pseudomonas aeruginosa without supporting evidence. Further complicating the utility of fosfomycin is the specified method for MIC determination, namely, agar dilution, which is not widely available and is both labor and time intensive. We therefore sought to determine the susceptibility of a large international collection of P. aeruginosa isolates (n = 198) to fosfomycin and to compare testing agreement rates across four methods: agar dilution, broth microdilution, disk diffusion, and Etest. Results were interpreted according to CLSI E. coli breakpoints, with 49.0 to 85.8% considered susceptible, dependent upon the testing method used. Epidemiological cutoff values were calculated and determined to be 256 mu g/ml and 512 mu g/ml for agar dilution and broth microdilution, respectively. Agreement rates were analyzed using both agar dilution and broth microdilution with a resulting high essential agreement rate of 91.3% between the two susceptibility testing methods. These results indicate that broth microdilution may be a reliable method for fosfomycin susceptibility testing against P. aeruginosa and stress the need for P. aeruginosa-specific breakpoints.
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Details
- Title
- Performance of Four Fosfomycin Susceptibility Testing Methods against an International Collection of Clinical Pseudomonas aeruginosa Isolates
- Creators
- Elizabeth C. Smith - University of MinnesotaHunter Brigman - University of MinnesotaJadyn C. Anderson - University of MinnesotaChristopher L. Emery - Indiana UniversityTiffany E. Bias - Hahnemann University HospitalPhillip J. Bergen - Monash UniversityCornelia B. Landersdorfer - Monash UniversityElizabeth B. Hirsch - University of Minnesota
- Publication Details
- Journal of clinical microbiology, v 58(10)
- Publisher
- Amer Soc Microbiology
- Number of pages
- 9
- Grant note
- Merck; Merck & Company University of Minnesota College of Pharmacy Melendy/Peters' research scholarship
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:000575858700008
- Scopus ID
- 2-s2.0-85091585898
- Other Identifier
- 991019330807404721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Microbiology