Journal article
Phenotypic Characterization of Streptococcus pneumoniae Biofilm Development
Journal of bacteriology, Vol.188(7), pp.2325-2335
01 Apr 2006
PMCID: PMC1428403
PMID: 16547018
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
ABSTRACT Streptococcus pneumoniae is among the most common pathogens associated with chronic otitis media with effusion, which has been hypothesized to be a biofilm disease. S. pneumoniae has been shown to form biofilms, however, little is known about the developmental process, the architecture, and the changes that occur upon biofilm development. In the current study we made use of a continuous-culture biofilm system to characterize biofilm development of 14 different S. pneumoniae strains representing at least 10 unique serotypes. The biofilm development process was found to occur in three distinct stages, including initial attachment, cluster formation, and biofilm maturation. While all 14 pneumococcal strains displayed similar developmental stages, the mature biofilm architecture differed significantly among the serotypes tested. Overall, three biofilm architectural groups were detected based on biomass, biofilm thickness, and cluster size. The biofilm viable cell counts and total protein concentration increased steadily over the course of biofilm development, reaching ∼8 × 108 cells and ∼15 mg of protein per biofilm after 9 days of biofilm growth. Proteomic analysis confirmed the presence of distinct biofilm developmental stages by the detection of multiple phenotypes over the course of biofilm development. The biofilm development process was found to correlate not only with differential production of proteins but also with a dramatic increase in the number of detectable proteins, indicating that biofilm formation by S. pneumoniae may be a far more complex process than previously anticipated. Protein identification revealed that proteins involved in virulence, adhesion, and resistance were more abundant under biofilm growth conditions. A possible role of the identified proteins in biofilm formation is discussed.
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Details
- Title
- Phenotypic Characterization of Streptococcus pneumoniae Biofilm Development
- Creators
- Magee Allegrucci - Department of Biological Sciences, Binghamton University, Binghamton, New YorkF. Z Hu - Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PennsylvaniaK Shen - Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PennsylvaniaJ Hayes - Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PennsylvaniaGarth D Ehrlich - Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, Pennsylvania, Department of Microbiology and Immunology, Drexel University, College of Medicine, Pittsburgh, PennsylvaniaJ. Christopher Post - Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PennsylvaniaKarin Sauer - Department of Biological Sciences, Binghamton University, Binghamton, New York
- Publication Details
- Journal of bacteriology, Vol.188(7), pp.2325-2335
- Publisher
- American Society for Microbiology (ASM)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Identifiers
- 991014878227104721
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- Web of Science research areas
- Microbiology