Journal article
Phospholipase D hydrolyzes short-chain analogs of phosphatidylcholine in the absence of detergent
Lipids, v 33(2)
Feb 1998
PMID: 9507245
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Phospholipase D is an important enzyme in signal transduction in neuronal tissue. A variety of assays have been used to measure phospholipase D activity in vitro. The most typical measure of phospholipase D activity is the production of phosphatidylethanol in the presence of ethanol. Phosphatidylethanol is a product of transphosphatidylation activity that is considered a unique property of phospholipase D. To support transphosphatidylation activity, high concentrations of ethanol may be required. Furthermore, most assays in the literature utilize a detergent. These extreme conditions, detergent and ethanol, may alter phospholipase D and hinder the study of its regulation. In this manuscript we describe an assay that eliminates these potentially confounding conditions. It utilizes high specific activity [3H]butanol as a nucleophilic receptor. This eliminates the need for high concentrations of alcohol. The substrate is an analog of phosphatidylcholine that contains short-chain fatty acids, 1,2-dioctanoyl-sn-glycero-3-phosphocholine. Phospholipase D readily hydrolyzes this substrate in the absence of detergent. This novel assay should be useful in the further characterization of phospholipase D.
Metrics
Details
- Title
- Phospholipase D hydrolyzes short-chain analogs of phosphatidylcholine in the absence of detergent
- Creators
- Leslee Davis - Department of Pharmacology MCP Hahnemann School of Medicine Allegheny University of the Health Sciences 3200 Henry Ave 19129 Philadelphia PAJeffrey Maglio - Department of Pharmacology MCP Hahnemann School of Medicine Allegheny University of the Health Sciences 3200 Henry Ave 19129 Philadelphia PAJoel Horwitz - Department of Pharmacology MCP Hahnemann School of Medicine Allegheny University of the Health Sciences 3200 Henry Ave 19129 Philadelphia PA
- Publication Details
- Lipids, v 33(2)
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000072119600013
- Scopus ID
- 2-s2.0-0031906689
- Other Identifier
- 991014877869304721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Nutrition & Dietetics