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Phosphoproteins altered by antiproliferative doses of human interferon-β in a human bladder carcinoma cell line
Journal article   Peer reviewed

Phosphoproteins altered by antiproliferative doses of human interferon-β in a human bladder carcinoma cell line

Gerald Soslau, Alfred R. Bogucki, David Gillespie and Howard R. Hubbell
Biochemical and biophysical research communications, v 119(3), pp 941-948
30 Mar 1984
DOI: https://doi.org/10.1016/0006-291X(84)90864-7
PMID: 6424675
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Phosphoproteins of control and IFN-β treated human bladder carcinoma cells (RT4) were labelled in vitro with [32P]-ATP and analyzed by polyacrylamide gel electrophoresis. Cells treated with antiproliferative doses of IFN had reduced levels of phosphorylated 60 Kd and 40 Kd proteins. IFN also induced within 24 hours the modification of a low molecular weight phosphoprotein doublet in the 22–24,000 molecular weight range. The ability to phosphorylate high molecular weight proteins by the in vitro procedures was generally depressed by IFN treatment. There was a dramatic shift in the phosphorylation of alkali stable phosphoamino acids associated with proteins in the 43–50,000 molecular weight range in IFN-treated cells. Preliminary studies indicate that at least some of the IFN-induced modifications of cellular phosphoproteins may result from transcriptional control of specific oncogenes.

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Web of Science research areas
Biochemistry & Molecular Biology
Biophysics
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