Logo image
Back
Phosphorylation of Caspase-9 in the Cytosolic Fraction of the Cerebral Cortex of Newborn Piglets Following Hypoxia
Journal article   Open access   Peer reviewed

Phosphorylation of Caspase-9 in the Cytosolic Fraction of the Cerebral Cortex of Newborn Piglets Following Hypoxia

Yosef Levenbrown, Qazi M. Ashraf, Nicoletta Maounis, Om P. Mishra and Maria Delivoria – Papadopoulos
Neuroscience letters, v 447(1)
01 Oct 2008
DOI: https://doi.org/10.1016/j.neulet.2008.09.076
PMID: 18840507
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://europepmc.org/articles/pmc2657363View
Accepted (AM)Open Access (License Unspecified),  Open

Abstract

Apoptosis Brain Caspase-9 Hypoxia Newborn
We have previously shown that hypoxia leads to increased expression and increased activity of caspase-9 in the cerebral cortex of newborn piglets. Previous studies have demonstrated the importance of caspase-9 in the initiation of the apoptotic cascade, however, the mechanism of caspase-9 activation is not well understood. Experiments were conducted on newborn piglets 2 – 3 days of age that were anesthetized and mechanically ventilated. Hypoxia was induced by lowering the Fi0 2 to 0.05 – 0.07 × 1 hour, and was confirmed biochemically by demonstrating decreased levels of ATP and Pcr in the hypoxic groups in comparison with the normoxic group. The ATP level was 1.99±0.66 in the hypoxic group versus 4.10±0.19 in the normoxic group, P < 0.05, and the PCr value was 0.68±0.14 in the hypoxic group, compared to 2.98±0.39 in the normoxic group, p< 0.05. The cytosol of the neuronal nuclei from the cerebral cortex was probed with anti-phosphorylated Ser 196 caspase – 9 antibody, using Western blot analysis. Protein bands were analyzed using image densitometry. In both the hypoxic and normoxic samples, protein bands were demonstrated just above the 50 kD marker. Phosphorylated caspase – 9 expression in OD × mm 2 was 43.85 ± 8.4 in the normoxic group and 67.6 ± 9.88 in the hypoxic group, P < 0.05. The results of this study demonstrate that caspase-9, a key protein in hypoxia induced apoptosis, is phosphorylated at the Ser 196 site during hypoxia. The results demonstrate that hypoxia results in a post-translational modification of caspase-9 at Ser 196 , which may alter the activity of caspase-9 in the hypoxic newborn brain.

Metrics

5 Record Views
4 citations in Web of Science
4 citations in Scopus

Details

UN Sustainable Development Goals (SDGs)

This publication has contributed to the advancement of the following goals:

#3 Good Health and Well-Being

InCites Highlights

Data related to this publication, from InCites Benchmarking & Analytics tool:

Web of Science research areas
Neurosciences
Logo image