Journal article
Phosphorylation of the insect immunophilin FKBP46 by the Spodoptera frugiperda homolog of casein kinase II
Gene, v 246(1), 169
04 Apr 2000
PMID: 10767538
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Immunophilins are a family of conserved proteins found in both prokaryotes and eukaryotes, that exhibit peptidylprolyl isomerase (PPIase) activity. Members of this family bind to immunosuppressive drugs and on this basis are divided into two classes: FKBPs bind to FK506 and rapamycin, while cyclophilins bind to cyclosporin A. In this paper, we report on insect immunophilin FKBP46 and its associated kinase. The insect FKBP46 belongs to the high-molecular-weight immunophilins and shares many characteristic features with its mammalian counterparts, but its functional role remains unclear. Here, we show that FKBP46 is phosphorylated by a protein kinase present in the nucleus of both insect
Spodoptera frugiperda (Sf9) and human Jurkat cells. This protein kinase is immunoreactive with polyclonal antiserum raised against
Drosophila melanogaster casein kinase II (CKII). We have cloned, overexpressed and characterized a new member of the CKII family derived from
Spodoptera frugiperda cells. Recombinant Sf9 CKII α subunit shares 75% identity to human, chicken and
Drosophila melanogaster homologs, whereas the Sf9 CKII β subunit is 77% identical to rat, chicken and human. Moreover, we demonstrate that the insect immunophilin FKBP46 can be phosphorylated by human and Sf9 casein kinase II. Finally, we show that FKBP46 interacts with DNA, and this interaction is not prevented by phosphorylation.
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Details
- Title
- Phosphorylation of the insect immunophilin FKBP46 by the Spodoptera frugiperda homolog of casein kinase II
- Creators
- Andrzej Steplewski - Thomas Jefferson UniversityWolfgang Ebel - Thomas Jefferson UniversitySonia L. Planey - Thomas Jefferson UniversityEmad S. Alnemri - Thomas Jefferson UniversityNoreen M. Robertson - Thomas Jefferson UniversityGerald Litwack - Thomas Jefferson University
- Publication Details
- Gene, v 246(1), 169
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- SOM Dean - Research Administration
- Web of Science ID
- WOS:000086490600017
- Scopus ID
- 2-s2.0-0034603829
- Other Identifier
- 991021463429604721
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- Web of Science research areas
- Genetics & Heredity